浙江省桂花叶斑病的病原鉴定及生物学特性等相关研究  

Identification and biological characteristics of phytopathogen in Osmanthus fragrans in Zhejiang Province of China

作　　者：王士臻 黄俊[2] 李明江 黄英杰 张娟 WANG Shizhen;HUANG Jun;LI Mingjiang;HUANG Yingjie;ZHANG Juan(Zhejiang Institute of Landscape Plants and Flowers,Hangzhou 311200,China;Institute of Plant Protection and Microbiology,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China)

机构地区：[1]浙江省园林植物与花卉研究所,浙江杭州311200 [2]浙江省农业科学院植物保护与微生物研究所,浙江杭州310021

出　　处：《浙江农业学报》2024年第12期2763-2773,共11页Acta Agriculturae Zhejiangensis

基　　金：国家重点研发计划项目(2021YFD1000500)。

摘　　要：近年来,随着桂树种植范围的推广且种植品种单一,桂花叶斑病的发生日趋严重,为明确杭州市内引起该病害发生的病原菌种类及其生物学特性,本研究首先通过发病部位组织分离培养获得其致病菌株一株,并通过ITS序列分析鉴定病原菌为葡萄座腔菌Botryosphaeria dothidea。其次,进一步探究了温度、pH值和培养基等不同环境条件对B.dothidea生长的影响,最终明确其适宜生长温度范围为23~28℃,最适生长pH值为5,最适生长培养基为燕麦培养基(OMA),不同碳、氮源对其生长影响不显著。同时通过不同温度梯度明确其致死温度及其致病能力,结果表明,其致死温度为55℃、20 min,致病能力随温度的升高明显增强。最后,通过药剂抑菌实验,完成5种药剂抑菌能力评价,其中抑菌最显著的是稀释10000倍的20%丙环唑微乳剂,抑菌率超过90%。Osmanthus fragrans,an evergreen tree with tremendous economic and ornamental value,has been extensively planted around the world.However,the disease of leaf spot seriously affected the quality and safety of Osmanthus fragrans due to uniform variety cultivation in Hangzhou.In this study,we isolated one strain that caused leaf spots and identified the fungi as Botryosphaeria dothidea by ITS sequence analysis.To clarify different factors that influence the growth of Botryosphaeria dothidea,we set different temperatures,pH values,culture media,and carbon and nitrogen sources,and the result showed its optimum temperature range was 23-28℃,the optimum pH value was 5,and the optimum culture medium was OMA.The growth of B.dothidea showed no obvious difference between the tested carbon and nitrogen sources.Besides,the pathogenicity was getting intense with the temperature rising and the lethal temperature was 55℃.The indoor antifungal test showed an inhibition rate of 20%propiconazole microemulsion was above 90%.

关 键 词：桂花叶斑病 葡萄座腔菌 生物学特性 

分 类 号：S436.85[农业科学—农业昆虫与害虫防治]