红景天苷对砷中毒大鼠心功能的抗细胞凋亡作用  

Anti apoptotic effect of salidroside on heart function in arsenic poisoned rats

作　　者：田芮萌 秦文娟 TIAN Ruimeng;QIN Wenjuan(Department of Ultrasound,Anning First People's Hospital,Anning 650300,China;Department of Ultrasound,the First Affiliated Hospital of Shihezi University,Shihezi 832008,China)

机构地区：[1]安宁市第一人民医院超声科,云南安宁650300 [2]石河子大学第一附属医院超声医学科,新疆石河子832008

出　　处：《中国医学影像技术》2025年第2期212-218,共7页Chinese Journal of Medical Imaging Technology

摘　　要：目的观察红景天苷对砷中毒大鼠心功能的抗细胞凋亡作用。方法将40只雄性大鼠随机分为低剂量红景天苷(Sal-L)组、高剂量红景天苷(Sal-H)组、砷中毒组(SA组)、无砷组(non-SA组)及对照组,每组各8只。予Sal-L组、Sal-H组及SA组自由饮用SA溶液[10 mg/(kg·d)]30天,non-SA组和对照组自由饮用无菌蒸馏水30天;分别对Sal-L组、Sal-H组及non-SA组以红景天苷溶液15、30及30 mg/(kg·d),对SA组及对照组以等量生理盐水连续灌胃21天。完成超声心动图检查后取心肌组织行HE染色和TUNEL凋亡分析,测定凋亡相关蛋白含量,观察红景天苷对砷中毒大鼠心功能的改善作用。结果5组左心室短轴缩短率、左心室射血分数和峰值应变差异均有统计学意义(P均<0.05),基本符合SA组<Sal-L组<Sal-H组<non-SA组=对照组(P均<0.05)。砷中毒致大鼠心肌细胞受损、凋亡细胞数目增加,重组人体B淋巴细胞瘤-2相关X蛋白水平上升、重组人体B淋巴细胞瘤-2蛋白水平下降;经红景天苷干预后好转,尤以Sal-H组为著。结论红景天苷可通过抗细胞凋亡作用保护砷中毒大鼠心功能,且具有剂量依赖性。Objective To observe the anti apoptotic effect of salidroside on heart function in arsenic poisoned rats.Methods Forty male rats were randomly divided into low-dose salidroside group(Sal-L group),high-dose salidroside group(Sal-H group),sodium arsenite(SA)poisoning group(SA group),non-SA group and control group(each n=8).Rats in Sal-L,Sal-H and SA groups were given SA solution(10 mg/[kg·d]),while in non-SA and control groups were given sterile distilled water for 30 days.Then,rats in Sal-L,Sal-H and non-SA groups underwent gavage with salidroside solution at doses of 15,30 and 30 mg/(kg·d),while those in SA and control groups underwent gavage with physiological saline for consecutive 21 days.Echocardiogram was performed,then myocardial tissue was obtained for HE staining and TUNEL apoptosis analysis,and the content of apoptosis related proteins were measured.The improvement effect of salidroside on heart function in arsenic poisoned rats was evaluated.Results Significant differences of left ventricular fraction shortening,left ventricular ejection fraction and peak strain were found among 5 groups(all P<0.05),basically presented as SA group<Sal-L group<Sal-H group<non-SA group=control group(all P<0.05).After arsenic poisoning,myocardial cells were damaged,apoptotic cells increased,B-cell lymphoma 2 associated X protein increased but B-cell lymphoma 2 protein decreased.After intervention with salidroside,improvement of the above situation was observed,especially in Sal-H group.Conclusion Salidroside could protect heart function in arsenic poisoned rats through anti apoptotic effect,with certain dose dependence.

关 键 词：大鼠 砷中毒 红景天苷 超声检查 

分 类 号：R595.2[医药卫生—内科学] R445.1[医药卫生—临床医学]