基于丝网印刷碳电极/还原氧化石墨烯/金纳米颗粒复合物的电化学传感器检测新型冠状病毒抗体  

Detection of COVID-19 antibody by electrochemical sensor based on SPCE/rGO/AuNPs

作　　者：陈晓雯 王钰结 张晓敏 蓝婉莹 黄晓霞 霍朝晖 CHEN Xiaowen;WANG Yujie;ZHANG Xiaomin;LAN Wanying;HUANG Xiaoxia;HUO Zhaohui(School of Chemistry and Materials Science,Guangdong University of Education,Guangzhou 510303,China;Engineering Technology Development Center of Advanced Materials&Energy Saving and Emission Reduction in Guangdong Colleges and Universities,Guangzhou 510303,China)

机构地区：[1]广东第二师范学院化学与材料科学学院,广州510303 [2]广东省普通高校先进材料与节能减排工程技术开发中心,广州510303

出　　处：《分析试验室》2025年第1期102-107,共6页Chinese Journal of Analysis Laboratory

基　　金：大学生创新创业训练计划项目(S202314278017);广东省普通高校特色创新项目(2022KTSCX090);广州市基础研究计划基础与应用基础研究项目(202102020424)资助。

摘　　要：本文通过原位生长法合成还原氧化石墨烯/金纳米颗粒(rGO/AuNPs)复合材料,采用扫描电子显微镜,X-射线衍射谱,X-射线光电子能谱和紫外-可见吸收光谱对rGO/AuNPs材料进行表征。以丝网印刷碳电极(SPCE)为基底电极,rGO/AuNPs为电极修饰材料,利用AuNPs与抗原形成Au-S键的自组装方式固定新型冠状病毒(COVID-19)抗原,构建了一种简单的电化学免疫传感器,并用于检测COVID-19抗体。结果显示,该传感器检测COVID-19抗体的线性范围为500 fg/mL~100 pg/mL,检出限为1.63 fg/mL。In this paper,reduced graphene oxide/gold nanoparticles(rGO/AuNPs)composite was synthesized by in-situ growth method.Then,the rGO/AuNPs was characterized by scanning electron microscopy,X-ray diffraction pattern,X-ray photoelectron spectroscopy and ultraviolet-visible spectrometry.A simple electrochemical immunosensor for the detection of Corona virus disease 2019(COVID-19)antibody was constructed by using screenprinted carbon electrode(SPCE)as the based electrode and rGO/AuNPs as the electrode modification material and forming Au-S bond by self-assembly of AuNPs with antigen to immobilize the COVID-19 antigen.The results showed the COVID-19 antibody was detected in a linear range of 500 fg/mL-100 pg/mL,and the detection limit was 1.63 fg/mL.

关 键 词：石墨烯 纳米金 COVID-19抗体检测 电化学传感器 

分 类 号：O657.1[理学—分析化学] R446.6[理学—化学]