上转发光技术在鼠疫疫情监测现场快速检测实用性评价  

The practicability of up-converting phosphor technology in the field of plague epidemic surveillance

作　　者：张海鹏[1] 杨丰义 段存娟 彭海燕[1] 石丽媛[1] 王鹏[1] 钟佑宏[1] Zhang Haipeng;Yang Fengyi;Duan Cunjuan;Peng Haiyan;Shi Liyuan;Wang Peng;Zhong Youhong(Yunnan Provincial Key Laboratory for Zoonosis Control and Prevention,Yunnan Institute for Endemic Disease Control and Prevention,Dali 671000,China)

机构地区：[1]云南省地方病防治所云南省自然疫源性疾病防控技术重点实验室,大理671000

出　　处：《中华地方病学杂志》2024年第9期755-759,共5页Chinese Journal of Endemiology

基　　金：国家自然科学基金(81160354);云南省科技创新人才培养对象(202305AD160011);云南省医学学科后备人才培养对象(H-2019003)

摘　　要：目的评价上转发光技术在鼠疫疫情监测现场快速检测的实用价值,为其在鼠疫疫情监测现场工作中的推广和应用提供科学依据。方法2020年9月,在云南省勐海县鼠疫疫情现场共收集116份样本(包含疫情判定样本4份),其中人体血液、淋巴液样本24份,鼠血液样本9份,肝脏、肌肉样本83份;2023年3月,丽江市鼠疫疫情现场监测共收集鼠肝脏及肌肉样本12份(均为疫情判定样本)。上述样本均使用上转发光技术进行鼠疫耶尔森菌(以下简称鼠疫菌)抗体、抗原检测。疫情判定样本在使用上转发光技术检测的同时,进行血凝试验、实时荧光定量PCR及细菌培养,比较不同实验方法检测过程及结果,分析上转发光技术检测鼠疫菌的优缺点,判定该检测方法在鼠疫疫情监测现场中优先使用的可行性。结果勐海县鼠疫疫情样本经上转发光技术检测,鼠疫菌阳性结果19份(抗体阳性1份、抗原阳性18份)。疫情判定样本中,经上转发光技术检测,鼠疫菌阳性结果样本为4份,其血凝试验、实时荧光定量PCR与细菌培养结果均为阳性。丽江市鼠疫疫情样本经上转发光技术检测,鼠疫菌阳性结果样本为2份(抗原阳性),其血凝试验和实时荧光定量PCR结果均为阳性,细菌培养结果阳性1份。上转发光技术、血凝试验、实时荧光定量PCR、细菌培养所需时长分别为0.5、4.0、2.5、72.0 h。结论上转发光技术检测鼠疫菌结果与血凝试验、实时荧光定量PCR及细菌培养结果基本相符,且用时较短。当样本数量较多时,该方法可在鼠疫疫情监测现场工作中优先使用,快速完成鼠疫疫情初判,为下一步的鼠疫防控工作节约时间及经济成本。Objective To evaluate the practical value of the up-converting phosphor technology(UPT)in the field fast detection of plague,and to provide scientific basis for its promotion and application in the field work of plague monitoring.Methods In September 2020,a total of 116 samples(including 4 samples for epidemic determination)were collected at the plague epidemic site in Menghai County,Yunnan Province,including 24 human blood and lymphatic fluid samples,83 rat liver and muscle samples,and 9 rat blood samples.In March 2023,a total of 12 rat liver and muscle samples were collected from Lijiang City for on-site monitoring of plague outbreak(all of them were outbreak determination samples).All of the above samples were tested for Yersinia pestis antibody and antigen using the up-converting phosphor technology.At the same time,haemagglutination test,real-time fluorescence quantitative PCR and bacterial culture were conducted to compare the detection process and results of different experimental methods,the advantages and disadvantages of the up-converting phosphor technology for detecting Yersinia pestis were analyzed,and the feasibility of using this detection method in the field of plague epidemic monitoring was judged.Results The plague epidemic samples site in Menghai County,Yunnan Province were tested by up-converting phosphor technology,and 19 samples were found to be positive for Yersinia pestis(1 antibody-positive and 18 antigen-positive).Among the samples determined,4 samples with positive results of Yersinia pestis were detected by up-converting phosphor technology,and the results of their haemagglutination test,real-time fluorescence quantitative PCR and bacterial culture were all positive.All samples from Lijiang City were tested by up-converting luminescence technology,two samples were positive for Yersinia pestis(antigen-positive).The results of haemagglutination test and real-time fluorescence quantitative PCR were positive,and one sample was positive for bacterial culture.The time required for up-convert

关 键 词：上转发光技术 鼠疫 疫情 现场应用 

分 类 号：R516.8[医药卫生—内科学]