锑铈催化分光光度法检测尿碘的方法研究  

A method for detecting urine iodine by antimony cerium catalytic spectrophotometry

作　　者：徐署东 谢继安[1] 丁刚[2] 许娴[3] 李卫东[2] Xu Shudong;Xie Ji'an;Ding Gang;Xu Xian;Li Weidong(Department of Physical and Chemical laboratory,Anhui Center for Disease Control and Prevention,Hefei 230601,China;Office of the Center,Anhui Center for Disease Control and Prevention,Hefei 230601,China;Department of Endemic and Parasitic Disease,Anhui Center for Disease Control and Prevention,Hefei 230601,China)

机构地区：[1]安徽省疾病预防控制中心理化检验室,合肥230601 [2]安徽省疾病预防控制中心办公室,合肥230601 [3]安徽省疾病预防控制中心地方病与寄生虫病防治科,合肥230601

出　　处：《中华地方病学杂志》2024年第9期750-754,共5页Chinese Journal of Endemiology

基　　金：中国疾控中心公共卫生领域卫生健康标准化前期研究项目(BZ2023-Q003)

摘　　要：目的建立尿碘的锑铈催化分光光度检测方法(以下简称新方法)。方法利用碘对锑铈氧化还原反应的高效催化能力,在0~300μg/L尿碘质量浓度范围内,应用新方法开展拟合曲线的线性关系以及方法检出限、精密度、准确度等实验,与国家卫生行业标准推荐的砷铈催化分光光度法(WS/T 107.1-2016)进行方法对比实验。结果在0~300μg/L尿碘质量浓度范围内,拟合曲线相关系数│r│均>0.9990,定性检出限为7.96μg/L(取样量为0.25 ml);选取4种碘质量浓度的实际尿样进行精密度实验,检测精密度相对标准偏差(RSD)在0.96%~2.87%,均<5%;实际尿样加标回收率范围为99.62%~103.88%,总平均回收率为101.75%;两种方法分别检测30份尿样,结果比较差异无统计学意义(t=0.68,P=0.501)。结论新方法反应灵敏度高,准确性好,避免了使用剧毒物质三氧化二砷,所用的锑试剂安全性高,废液排放环境污染小,检测步骤与砷铈催化分光光度法类似,实验室原有尿碘检测人员无需进行特殊培训即可操作使用,适合基层碘缺乏病监测工作中尿碘检测的应用推广。Objective To establish a new method for antimony cerium catalytic spectrophotometric detection(referred to as the new method)of urinary iodine.Methods By utilizing the efficient catalytic ability of iodine for the oxidation-reduction reaction of antimony and cerium,a new method was applied to conduct linear relationship fitting curve,detection limit,pecision,accuracy and other experiments within the range of 0-300μg/L urine iodine mass concentration.The method was compared with the Arsenic Cerium Catalytic Spectrometric Method(WS/T 107.1-2016)recommended national industry standard.Results In the range of 0-300μg/L urine iodine mass concentration,the correlation coefficients讦r讦of the fitted curves were>0.9990,and the qualitative detection limit was 7.96μg/L(with a sampling volume of 0.25 ml).Four actual urine samples with different iodine mass concentrations were selected for precision experiments,and the relative standard deviation(RSD)of the detection precision ranged from 0.96%to 2.87%,all<5%.The actual urine sample spiked recovery range from 99.62%to 103.88%,with an overall average recovery rate of 101.75%.Two methods were used to detect 30 urine samples,and the difference in results was not statistically significant(t=0.68,P=0.501).Conclusions The new method has high reaction sensitivity and accuracy,avoiding the use of highly toxic substance arsenic trioxide.The antimony reagent used is safe,and the environmental pollution caused by waste liquid discharge is small.The detection steps are similar to Arsenic Cerium Catalytic Spectrometric Method,the laboratory urine iodine detection personnel do not need special training to operate and use it,which is suitable for the application and promotion of urine iodine detection in grassroots iodine deficiency disorders monitoring work.

关 键 词：尿 碘 锑铈催化 分光光度法 

分 类 号：R446.12[医药卫生—诊断学]