盐碱胁迫对密本南瓜种子萌发及幼苗生长的影响  

Effects of Salt-alkali Stress on Seed Germination and Seedling Growth of Cucurbita moschata Duch

作　　者：黎建玲 方振名 黄宁 黄小范 乔清华 Li Jianling;Fang Zhenming;Huang Ning;Huang Xiaofan;Qiao Qinghua(School of Biology and Pharmacy,Key Laboratory of Mountain Biodiversity Conservation,Education Department of Guangxi Zhuang Autonomous Region,Yulin Normal University,Yulin 537000;School of Smart Agriculture,Yulin Normal University,Yulin Guangxi 537000;School of Biology and Pharmacy,Student of Biologic Science 2016,Yulin Normal University,Yulin Guangxi 537000)

机构地区：[1]玉林师范学院生物与制药学院广西高校山地生物多样性保育重点实验室,广西玉林537000 [2]玉林师范学院智慧农业学院,广西玉林537000 [3]玉林师范学院生物与制药学院,广西玉林537000

出　　处：《玉林师范学院学报》2024年第3期51-59,共9页Journal of Yulin Normal University

基　　金：广西科技计划项目(AD20159006);广西高校中青年教师科研基础能力提升项目(2022KY0574)

摘　　要：采用不同浓度中性盐(NaCl)、碱性盐(Na_(2)CO_(3))和盐碱混合溶液(NaCl+Na_(2)CO_(3))处理密本南瓜种子,研究盐碱胁迫对密本南瓜种子萌发及幼苗生长的影响,旨在为盐碱地区的耐盐南瓜的选育及栽培提供理论基础.结果表明:3组不同浓度盐碱胁迫对密本南瓜种子的萌发及幼苗根的生长均有抑制作用.具体表现为:随着NaCl、Na_(2)CO_(3)处理浓度的升高,密本南瓜种子发芽率、发芽势、发芽指数、活力指数均呈逐渐下降趋势,而混合盐处理下各萌发指标则呈先上升后下降趋势.NaCl浓度≤50 mmoL·L^(-1)、混合盐(NaCl+Na_(2)CO_(3))浓度≤(25+10)mmoL·L^(-1)胁迫对密本南瓜种子萌发和幼苗生长无显著的抑制作用.高浓度的三组盐碱胁迫对南瓜种子萌发均产生显著抑制作用,且碱性盐、混合盐胁迫处理比中性盐胁迫处理对密本南瓜种子的盐害影响更加明显.主根长、侧根数和主根的鲜质量及干质量均随3组盐碱胁迫浓度的增大而呈下降趋势.在Na_(2)CO_(3)浓度≥50 mmoL·L^(-1)、混合盐浓度≥(125+50)mmoL·L^(-1)胁迫下的密本南瓜幼苗根部受到严重毒害,导致根部腐烂而无法生长.复萌试验时解除NaCl胁迫后复萌率较高.说明密本南瓜种子可以抵御低浓度中性盐和低浓度盐碱的侵害,对盐碱胁迫具有一定的微耐性.This study used different concentrations of neutral salt(NaCl),alkaline salt(Na_(2)CO_(3)),and salt-alkali mixed solution(NaCl+Na_(2)CO_(3))to treat seeds of Cucurbita moschata Duch,and investigated the effects of salt-alkali stress on seed germination and seedling growth with the purpose to provide a theoretical basis for the selection and cultivation of salt tolerant Cucurbita moschata Duch in salt-alkali areas.The results showed that three different concentrations of salt alkali stress had inhibitory effects on the germination of seeds and the growth of seedling roots.Specifically,as the concentration of NaCl and Na_(2)CO_(3)treatment increases,the germination rate,germination potential,germination index,and vitality index of Cucurbita moschata Duch gradually decrease.However,after mixed salt treatment,each germination index shows a trend of first increasing and then decreasing.The stress of NaCl concentration≤50 mmol·L^(-1)and mixed salt(NaCl+Na_(2)CO_(3))concentration≤(25+10)mmol·L^(-1)had no significant inhibitory effect on the germination of seeds and seedling growth.The three groups of salt-alkali stresses with High concentrations significantly inhibited the germination of seeds,and alkaline salt and mixed salt stress treatments had a more pronounced effect on salt damage to the seeds than neutral salt stress treatments.The length of the main root,the number of lateral roots,and the fresh and dry weight of the main root all showed a decreasing trend with the increase of salt alkali stress concentration in the three groups.Under the stress of Na_(2)CO_(3)concentration≥50 mmol·L^(-1)and mixed salt concentration≥(125+50)mmol·L^(-1),the roots of seedlings were se⁃verely damaged,resulting in root rot and inability to grow.The reemergence rate was higher after relieving NaCl stress dur⁃ing the reemergence experiment.This indicates that the seeds of Cucurbita moschata Duch can resist the invasion of low concentration neutral salts and low concentration salt-alkali stress,and have a certain tolera

关 键 词：密本南瓜 盐碱胁迫 种子萌发 幼苗生长 

分 类 号：Q945[生物学—植物学]