Identification and Determination of the Major Constituents in Xie-Bai-San by Ultra-performance Liquid Chromatography–Mass Spectrometry  

作　　者：Bao-Xin Li Peng-Yue Li Li-Xia Hao Xiao-Xia Xue Sha-Sha Kong Qin-He Zhao Yun-Tao Dai 

机构地区：[1]Traditional Chinese Medicine Analysis,Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing,China

出　　处：《World Journal of Traditional Chinese Medicine》2024年第3期334-346,共13页世界中医药杂志(英文)

基　　金：The National Key R&D Program of China (2023YFC2308200);Scientific and Technological Innovation Project of China Academy of Chinese Medical Sciences (No.CI2023E001TS)

摘　　要：Objective: The ancient classical Chinese formulation, Xie-Bai-San(XBS), was studied qualitatively and quantitatively, and its main components were characterized. Materials and Methods: The chemical compounds in XBS were identified using a combination of ultra-performance liquid chromatography-quadrupole-Orbitrap mass spectrometry and molecular networking(MN) analysis. In addition, a multiple reaction monitoring(MRM) quantification method was established using ultra-high-performance liquid chromatography–triple quadrupole mass spectrometry. Mulberroside A, kukoamine B, liquiritin, and glycyrrhizic acid were quantitatively analyzed in ten batches of XBS using this method. Results: Using clusters of MN and databases, 91 compounds were rapidly identified, including stilbene glycosides, saponins,fiavonoids, and alkaloids. Of these, 30, 33, and 28 compounds were from Mori Cortex, Lycii Cortex, and Glycyrrhizae Radix et Rhizoma,respectively. The MRM scanning mode was used for the simultaneous sensitive quantitative determination of multiple components in a wide range of complex samples. The four main components were quantified using this method, which has good linearity. Conclusions: This study provides a comprehensive and reliable method for the identification and quantitative analysis of XBS.

关 键 词：Chemical constituent identification molecular networking multiple reaction monitoring quantitative analysis Xie‑Bai‑San 

分 类 号：R286.0[医药卫生—中药学]