Pudilan Anti-inflammatory Oral Liquid and Organic Acid Component from Taraxaci Herba Attenuate Allergic Asthma in Young Mice Through Toll-like Receptor 2/Toll-like Receptor 4 Signaling Pathway  

作　　者：Yu-Zhi Mao Chen-Zi Li Wei-Quan Bu Bing Yang Ya-Ping Chen Jun Liu Jing Zhao E Sun Xiao-Bin Jia Liang Feng 

机构地区：[1]State Key Laboratory of Natural Medicines,School of Traditional Chinese Pharmacy,China Pharmaceutical University,Nanjing,China [2]College of Pharmacy,Xuzhou Medical University,Xuzhou,China [3]Third Clinical Medical College,Nanjing University of Chinese Medicine,Nanjing,China [4]Jumpcan Pharmaceutical Co.,Ltd.,Jiangsu,Taixing,China

出　　处：《World Journal of Traditional Chinese Medicine》2024年第3期294-302,共9页世界中医药杂志(英文)

基　　金：funded by the National Key R and D Program of China (2018YFC1706900, 2018YFC1706902);“Double First-Class” University project of China Pharmaceutical University (CPU2018GF07, CPU2018PZQ19);Jiangsu Provincial Administration of Traditional Chinese Medicine (YB201819);the Project Program of State Key Laboratory of Natural Medicines, China Pharmaceutical University (No. SKLNMZZ202025)

摘　　要：Objective: Allergic asthma(AA) is a chronic airway infiammatory disease characterized by airway hyper-responsiveness(AHR). Pudilan anti-infiammatory oral liquid(PDL), along with its main medicinal material, Taraxaci Herba(Taraxacum mongolicum Hand.-Mazz, TH),has been widely used to treat upper respiratory tract infections. Research has shown that the major ingredient of TH, the organic acid component(OAC), possesses favorable AA activity. However, the attenuated effects of PDL and OAC from TH(TH-OAC) on AA and their possible mechanisms remain poorly understood. This study analyzed the attenuating effects of PDL and TH-OAC on AA and the underlying mechanisms. Methods: Young BALB/c mice were sensitized and stimulated to develop asthma using ovalbumin. Histological examinations were performed by hematoxylin and eosin staining. Western blotting, immunohistochemistry, and protein expression detection of toll-like receptor 2(TLR2), TLR4, and orosomucoid 1-like protein 3(ORMDL3) were performed to detect the presence of infiammatory components in the lung tissue. The messenger RNA(mRNA) expression levels were determined using quantitative real-time polymerase chain reaction.Results: Results showed that PDL and TH-OAC alleviated augmented AHR and typical asthmatic pathological changes, including infiammatory infiltration and thickening of the alveolar wall. They also significantly reduced the levels of the immunoglobulin E, IL-4, IL-5, IL-6, tumor necrosis factor-α, and Nitric oxide(NO) in lung tissues of mice. Protein and mRNA expression levels of TLR2, TLR4, and ORMDL3 were downregulated following treatment with PDL and TH-OAC. Conclusions: PDL and TH-OAC can reduce asthma-induced infiammatory damage to the bronchi. These results provide a theoretical basis for the treatment of asthma in clinical settings.

关 键 词：Allergic asthma organic acid component Pudilan anti‑infiammatory oral liquid Taraxaci Herba toll‑like receptor 2 toll‑like receptor 4 

分 类 号：R285.5[医药卫生—中药学]