砂茴香HPLC特征图谱建立及不同部位化学模式识别研究  

Establishment of high performance liquid chromatography fingerprint of Ferula bungeana and chemical pattern recognition study of its different parts

作　　者：王青虎[1] 伊力奇 朝梁 王光明 宋亚伟 明慧 WANG Qinghu;YI Liqi;CHAO Liang;WANG Guangming;SONG Yawei;MING Hui(Mongolian Medical College,Inner Mongolia Minzu University,Tongliao 028000,China)

机构地区：[1]内蒙古民族大学蒙医药学院,内蒙古通辽028000

出　　处：《中草药》2024年第19期6749-6757,共9页Chinese Traditional and Herbal Drugs

基　　金：蒙药研发国家地方联合工程研究中心开放基金项目(MDK2023040);内蒙古自治区中蒙药重点实验室开放基金项目(MYX2022-K06)

摘　　要：目的 建立砂茴香Ferula bungeana的化学指纹图谱及定量分析方法,并比较其不同部位化学成分差异性,为其不同部位的功效和主治提供化学基础数据。方法 采用HPLC法建立15批砂茴香不同部位的化学指纹图谱,采用“中药色谱指纹图谱相似度评价系统”软件(2012年130723版),确定砂茴香不同部位各自的共有峰及其不同部位之间交叉共有峰。同时,采用对照品法指认砂茴香不同部位之间交叉共有峰,并进行主成分分析(principal component analysis,PCA)、偏最小二乘法判别分析(partial least squares discriminant analysis,PLS-DA)和方差分析,确认其潜在差异成分。采用HPLC法测定15批砂茴香不同部位中宽叶阿魏醇和伞形戊烯内酯的含量。结果 建立了15批砂茴香不同部位的化学指纹图谱,标定砂茴香全草共有峰为18个,而其地上部位和根中标定了共有峰各为16个。其各自共有峰相似度均大于0.9以上,说明砂茴香同一部位之间化学成分差异性较小。砂茴香不同部位之间交叉共有峰有7个,鉴定了其中1号峰为砂茴香碱A、8号峰为砂茴香酮A、15号峰为砂茴香素A、16号峰为宽叶阿魏醇和18号峰为伞形戊醇内酯。PCA、PLS-DA和方差分析结果显示,砂茴香不同部位之间的差异成分主要有3个,分别为砂茴香酮A、宽叶阿魏醇和伞形戊醇内酯,其VIP值分别为1.364、1.119和1.015。含量测定结果显示,宽叶阿魏醇和伞形戊醇内酯在15批砂茴香不同部位中含量变化分别为(2.235±0.025)~(9.481±0.133)mg/g和(6.670±0.067)~(28.820±0.346)mg/g,(0.694±0.010)~(1.958±0.025)mg/g和(0.138±0.002)~(1.784±0.020)mg/g,(5.113±0.077)~(13.550±0.176)mg/g和(9.2460±0.102)~(32.760±0.524)mg/g,说明不同来源砂茴香不同部位中宽叶阿魏醇和伞形戊醇内酯相对含量差异性较大。结论 从化学成分比较结果看,砂茴香不同部位之间存在一定的差异性,因此砂茴香不同部位药用价值各异具有合�Objective To establish a chemical fingerprint and quantitative analysis method for Ferula bungeana and compare the differences in chemical composition of its different parts,providing chemical basic data for the efficacy and treatment of different problems in its different parts.Methods HPLC method was used to establish the chemical fingerprint of different parts of 15 batches of F.bungeana.Then,the“similarity evaluation system for traditional Chinese medicine chromatographic fingerprints”software(2012 edition 130723)was used to determine the common peaks of different parts of F.bungeana and the cross common peaks between its different parts.At the same time,the reference method was used to identify the cross common peaks between different parts of F.bungeana,and their cross common peaks were subjected to principal component analysis(PCA),partial least squares discriminant analysis(PLS-DA),and analysis of variance to confirm their potential differential components.HPLC method was used to determine the content of conferol and umbelliprenin in different parts of 15 batches of F.bungeana.Results The chemical fingerprint were established for different parts of 15 batches of F.bungeana.Among 15 batches of the whole herb of F.bungeana,a total of 18 common peaks were identified,while 16 common peaks were identified in the aerial parts and roots of F.bungeana.The similarity of their respective common peaks was greater than 0.9,indicating that there was relatively little difference in chemical composition between the same part of F.bungeana.There were seven cross common peaks between different parts of F.bungeana,among which the first peak was identified as bungeanal alkaliod A,the 8th peak was identified as bungeanone A,the 15th peak was identified as bungeanin A,the 16th peak was identified as conferol and the 18th peak was ide ntified as umbelliprenin.The results of PCA,PLS-DA and analysis of variance showed that there were three main components with differences between different parts of F.bungeana,namely bungeano

关 键 词：砂茴香 高效液相色谱 化学指纹图谱 化学模式识别 宽叶阿魏醇 伞形戊烯内酯 

分 类 号：R286.2[医药卫生—中药学]