基于非靶向代谢组学的怀珍珠菊和野菊花化学成分差异性分析  

Non-targeted metabolomics study on differential chemical constituents of Chrysanthemum morifolium and Chrysanthemum indicum

作　　者：杨晓萌 赵楠[3] 刘欣欣[3] 张立新 张晓哲[3] 张丽华[3] YANG Xiaomeng;ZHAO Nan;LIU Xinxin;ZHANG Lixin;ZHANG Xiaozhe;ZHANG Lihua(School of Chemical Engineering,Shenyang University of Chemical Technology,Shenyang 110142,China;Shenyang Key Laboratory of Targeted Pesticides,Liaoning Province Key Laboratory of Green Functional Molecular Design and Development,Institute of Functional Molecules,Shenyang University of Chemical Technology,Shenyang 110142,China;CAS Key Laboratory of Separation for Analytical Chemistry,Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian 116023,China)

机构地区：[1]沈阳化工大学化学工程学院,辽宁沈阳110142 [2]沈阳化工大学功能分子研究所,辽宁省绿色功能分子设计与开发重点实验室,沈阳市靶向农药重点实验室,辽宁沈阳110142 [3]中国科学院分离分析化学重点实验室,中国科学院大连化学物理研究所,辽宁大连116023

出　　处：《中草药》2024年第19期6496-6507,共12页Chinese Traditional and Herbal Drugs

基　　金：辽宁省民生科技项目2021JH2/10200004;贵州省科技支撑项目(黔科合支撑[2020] 4Y218号)

摘　　要：目的 研究典型食用菊怀珍珠菊Chrysanthemum morifolium与典型药用菊野菊花C. indicum的差异性成分。方法采用UPLC/Q-TOF-MS结合非靶向代谢组学方法,筛选怀珍珠菊与野菊花的差异性成分。结果 从怀珍珠菊中共鉴定出包括黄酮类、萜类、苯丙素类、有机酸类等类化合物在内的80个代谢产物,其中木犀草苷、艾黄素、秦皮乙素等19个代谢产物是与野菊花具有显著差异性的分子。结论 萜类化合物为怀珍珠菊和野菊花的主要差异性代谢物,这些代谢物与抗炎、抑菌和抗虫害密切相关,值得进一步研究。Objective To study the differential components of typical edible chrysanthemum‘HZZJ’(Chrysanthemum morifolium)and typical medicinal chrysanthemum‘YJH’(C.indicum).Methods The differential components of‘HZZJ’and‘YJH’are screened by UPLC/Q-TOF-MS combined with non-target metabolomics method.Results A total of 80 metabolites including flavonoids,terpenoids,phenylpropanoids,organic acids and other compounds are identified in this study,of which 19,such as luteolin,artemisetin and aesculetin,are molecules with significant differences from wild chrysanthemums.Conclusion Terpenoids are the main differential metabolites of‘HZZJ’and‘YJH’,and these metabolites are closely related to anti-inflammatory,bacteriostatic and pest resistance,and deserve further study.

关 键 词：怀珍珠菊 野菊花 UPLC/Q-TOF-MS 主成分分析 差异性分析 萜类化合物 木犀草苷 艾黄素 秦皮乙素 

分 类 号：R284.1[医药卫生—中药学]