药用植物猴耳环的全长转录组测序分析  

Full-length Transcriptome Sequencing Analysis of Medicinal Plant Pithecellobium clypearia

作　　者：钟凤娣 凌腾泓 牟桂萍 朱孔宇 王鹏龙 闫晓东 ZHONG Feng-di;LING Teng-hong;MOU Gui-ping;ZHU Kong-yu;WANG Peng-long;YAN Xiao-dong(Guangdong Lingnanyuan Exploration and Design Co.,Ltd,Guangzhou 510095,Guangdong China;College of Forestry and Landscape Architecture,South China Agricultural University,Guangzhou 510642,Guangdong China;Guangdong Provincial Key Laboratory of Intelligent Port Security Inspection,Guangzhou 510700,Guangdong China;College of Environment and Chemical Engineering,Foshan University,Foshan 528000,Guangdong China;Technology Center of Huangpu Custom,Guangzhou 510730,Guangdong China)

机构地区：[1]广东省岭南院勘察设计有限公司,广东广州510095 [2]华南农业大学林学与风景园林学院,广东广州510642 [3]广东省口岸安全智能化检测重点实验室,广东广州510700 [4]佛山大学环境与化工学院,广东佛山528000 [5]黄埔海关技术中心,广东广州510730

出　　处：《亚热带植物科学》2024年第5期416-424,共9页Subtropical Plant Science

基　　金：广东省口岸安全智能化检测重点实验室项目(2023B1212010011)

摘　　要：利用PacBio Sequel测序技术对猴耳环Pithecellobium clypearia根部、成熟枝干、嫩枝、成熟叶片和嫩叶组织的混合样本进行全长转录组测序。成功鉴定出118 472个独特的转录本,进一步归纳为44 939个Unigenes。对Nr、Uniprot、GO、KEGG和KOG数据库进行比对,为37 198个Unigenes提供了功能注释,其中大部分Unigenes参与了生物学过程和代谢途径。同时,发现13 897个Unigenes经历了可变剪接,包括内含子保留、3'端剪接多样性和5'端剪接多样性。此外,还鉴定到377个和159个参与类黄酮和萜类化合物生物合成的结构基因以及132个和180个编码MYB和WRKY转录因子家族成员的Unigenes。研究结果扩展了猴耳环的基因组资源,为探索其生物活性成分的代谢调控机制、关键基因的筛选以及分子标记的开发提供了数据支持,为猴耳环药用价值的进一步开发奠定基础。As the plant with remarkable medicinal value,the insufficiency of genomic information of Pithecellobium clypearia restricts its in-depth comprehension.In this study,PacBio Sequel sequencing technology was employed to conduct full-length transcriptome sequencing of the mixed samples from roots,mature branches,young branches,mature leaves,and young leaf tissues of P.clypearia.The results showed that 118472 unique transcripts were successfully identified and further categorized into 44939 Unigenes.After comparison with the Nr,Uniprot,GO,KEGG,and KOG databases,functional annotations were provided for 37198 Unigenes,and the majority of these Unigenes were involved in biological processes and metabolic pathways.Simultaneously,13897 Unigenes were discovered to undergo alternative splicing,including intron retention,3'end splicing diversity,and 5'end splicing diversity.Additionally,377 and 159 structural genes involved in the biosynthesis of flavonoids and terpenoids,as well as 132 and 180 Unigenes encoding members of the MYB and WRKY transcription factor families,were identified.This study expanded the genomic resources of P.clypearia,provided valuable data support for exploring the metabolic regulatory mechanism of its bioactive components,screening of key genes,and development of molecular markers,laid a foundation for further exploitation of the medicinal value of P.clypearia in future.

关 键 词：猴耳环 全长转录组 类黄酮化合物 萜类化合物 可变剪接 

分 类 号：Q943[生物学—植物学]