气相色谱-三重四极杆质谱联用法同时快速测定果蔬中263种农药残留  

Simultaneous,rapid determination of 263 pesticide residues in fruits and vegetables using gas chromatography⁃triple quadrupole tandem mass spectrometry

作　　者：王君君 黎俊宏[1] 王文斌[2] 欧阳运富[1] 唐宏兵[1] 吴英[1] 刘华良 WANG Jun⁃jun;LI Jun⁃hong;WANG Wen⁃bin;OUYANG Yun⁃fu;TANG Hong⁃bing;WU Ying;LIU Hua⁃liang(Changzhou Municipal Center for Disease Control and Prevention,Jiangsu Changzhou 213001,China;不详)

机构地区：[1]常州市疾病预防控制中心,江苏常州213001 [2]常州市武进区疾病预防控制中心 [3]江苏省疾病预防与控制中心

出　　处：《江苏预防医学》2024年第5期562-564,587,共4页Jiangsu Journal of Preventive Medicine

基　　金：常州市科技项目应用基础研究计划中补助(CJ20220067);江苏省第六期“333高层次人才培养工程”第三层培养对象[(2022)3-4-163];常州市“十四五”卫生健康高层次人才培养工程-拔尖人才(2022CZBJ099)资助

摘　　要：目的建立气相色谱-三重四极杆质谱联用法同时快速测定果蔬中263种农药残留方法。方法样品前处理采用QuEChERS方法,经乙腈及萃取盐包萃取,净化盐包净化,氮吹浓缩,应用全扫描模式(Scan)和多反应监测模式(MRM)进行定性和外标法定量检测。结果263种农药检出限范围为0.003~1.251μg/kg,定量限范围为0.008~3.791μg/kg;在质量浓度为5~100μg/L的范围内呈现良好线性关系,决定系数R2均>0.99;平均加标回收率在83.8%~117.1%,相对标准偏差(RSDs)在0.2%~17.7%。采用建立方法对4种基质果蔬样品进行检测,共检出11种农药组分,均未超标。结论本次建立方法能同时快速检测果蔬中多种农药残留,为食品卫生日常监测、风险预警及应急检测提供保障。Objective To establish a gas chromatography⁃triple quadrupole tandem mass spectrometry for simultaneous,rapid determination of 263 pesticide residues in fruits and vegetables.Methods Fruit and vegetable sample were pretreated with the quick,easy,cheap,effective,rugged and safe(QuEChERS)method,extracted with acetonitrile and extraction salt package,purified with purification salt package and concentrated with nitrogen blow.Qualitative analysis was performed in a full scan mode and multiple reaction monitoring mode,and quantitative analysis was performed with the external standard method.Results The detection limit and limit of quantitation of 263 pesticides were 0.003 to 1.251μg/kg and 0.008 to 3.791μg/kg,respectively.Pesticides had a good linear relationship at a mass concentration of 5 to 100μg/L,with a correlation coefficient of>0.99,spiked recovery rates of 83.8%to 117.1%,and relative standard deviations of 0.2%to 17.7%.Gas chromatography⁃triple quadrupole tandem mass spectrometry detected 11 pesticide components in 4 matrix fruit and vegetable samples,which all did not exceed the standard.Conclusions The established gas chromatography⁃triple quadrupole tandem mass spectrometry is feasible for simultaneous,rapid determination of multiple pesticide residues in fruits and vegetables,which provides supports to daily monitoring,risk forecasting and emergency detection of food sanitation.

关 键 词：气相色谱-三重四极杆质谱联用仪 QUECHERS 蔬菜 水果 农药残留 

分 类 号：R113[医药卫生—公共卫生与预防医学]