Construction of a Multicellular Communication Network Model for Cell Co-Culture Technology and Evaluation of Its Simulation Capability  

作　　者：Yuan-Yuan Geng Chao Wei Guo-Fei Chen Bai-Xia Zhang 

机构地区：[1]Institute of Traditional Chinese Medicine,Hebei University,Baoding,China [2]Linyi Lanshan District Fangcheng Central Hospital,Linyi,China

出　　处：《World Journal of Traditional Chinese Medicine》2024年第4期465-475,共11页世界中医药杂志(英文)

基　　金：supported by the Hebei Province Natural Science Foundation of China (H2021201022);Youth Scienceof the National Natural Science Foundation of China (81903931);Post-graduates Innovation Fund Project of Hebei University (HBU2022ss001)

摘　　要：Objective:Cell co-culture technology has been widely used to analyze the effects of drugs on cell proliferation and the expression of some proteins in cells,especially in the field of traditional Chinese medicine(TCM);however,the interactions between cells and the transmission of TCM effects between cells have not been adequately studied.Materials and Methods:Using data on gene transcription regulation,biological response,signal channel,and cell-specific expression protein,we built a network for cell types based on entity grammar.Through the correspondence and location information of signal molecules and receptors,type-specific networks of single cells were connected and a multicellular network of smooth muscle cells,neurons,and vascular endothelial cells was constructed.The mechanism of action of nimodipine was analyzed based on the multicellular communication network and its simulation capability was evaluated.Results:The outputs generated by the model developed in this study showed that nimodipine inhibited smooth muscle contraction,due to the overload of Ca^(2+)and the toxicity of excitatory amino acids,and protected neurons and vascular endothelial cells by supporting cell proliferation and inhibiting cell apoptosis.These results were consistent with the known mechanism of nimodipine action,thus confirming that the multicellular network can be used to study the transmission of drug effects among cells.Conclusions:This study lays a foundation for the analysis of the transmission of drug effects in multi-cells,tissues,organs,and other spatial scales through multicellular co-culture experiments,based on a multicellular communication network.In addition,it provides a biological network model for the analysis of TCM action mechanisms.

关 键 词：Cell co‑culture cell type‑specific network drug action mechanism entity grammar system multi‑cell biological network model 

分 类 号：Q813.11[生物学—生物工程] R285[医药卫生—中药学]