感染轮状病毒婴幼儿的肠道菌群特征分析  

Characteristics of gut microbiota among infants with rotavirus infections

作　　者：谢楠 李岚[3] 程鑫田 刘凯洋 王凯 包小兵[2] XIE Nan;LI Lan;CHENG Xin⁃tian;LIU Kai⁃yang;WANG Kai;BAO Xiao⁃bing(Anyang Central Blood Station,Anyang Henan 455000,China;不详)

机构地区：[1]安阳市中心血站,河南安阳455000 [2]安阳市疾病预防控制中心 [3]安阳市妇幼保健院

出　　处：《江苏预防医学》2024年第6期690-693,704,共5页Jiangsu Journal of Preventive Medicine

基　　金：安阳市科技发展计划科技攻关项目(2023C01SF118)

摘　　要：目的了解婴幼儿感染轮状病毒后肠道菌群特征,初步揭示肠道菌群在轮状病毒感染中的作用。方法收集45例<3岁感染轮状病毒婴幼儿(病例组)和30例健康儿童(对照组)粪便标本。对其进行16S rRNA扩增子测序,对肠道菌群特征进行分析。通过生物信息学分析α多样性、β多样性、物种丰度差异,通过Lefse分析识别感染后可能的特异性菌种。结果α多样性5个指数2组差异均有统计学意义(Chao1,Observed species,Shannon,Simpson,Pielou′s evenness KW值分别为0、0、2×e^(-6)、2.5×e^(-5)、5.5×e^(-5),P值均<0.01)。与对照组相比,病例组α多样性显著性降低;β多样性存在显著性差异;在门水平上,厚壁菌门、拟杆菌门丰度升高,放线菌门丰度显著降低;在属水平上,双歧杆菌属、拟杆菌属、粪杆菌属的丰度显著降低,肠球菌属、链球菌属的丰度显著升高;在种水平上,普通拟杆菌、动物双歧杆菌丰度显著降低,唾液链球菌丰度显著升高;以上差异均有统计学意义(P值均<0.05)。肠球菌、肠杆菌、克雷伯氏杆菌、寡养单胞菌可能是感染轮状病毒婴幼儿粪便中的特异性菌。结论轮状病毒感染婴幼儿会导致患儿肠道菌群结构大幅改变,提示肠道菌群中特定差异的细菌种类在轮状病毒感染机制中可能起重要作用。Objective To investigate the characteristics of gut microbiota among infants with rotavirus infections,and to preliminarily unravel the role ofgut microbiota in rotavirus infections.Methods Fecal samples were collected from 45 rotavirus⁃infected infants under 3 years of age(case group)and 30 healthy children(control group).Stool samples were subjected to 16S rRNAamplicon sequencing,and the characteristics of gut microbiota were analyzed.The differences inα⁃diversity,β⁃diversityand species abundancewere compared between the case and control groups through bioinformatics analysis,and potential specific bacterial strains were identified post⁃infection using linear discriminant analysis Effect Size(LEfSe).Results There were significant differences in the fiveα⁃diversity metrics(Chao1index,observed species,Shannonindex,Simpsonindex,and Pielou′s evennessindexes were 0,0,2×e^(-6),2.5×e^(-5)and 5.5×e^(-5))between the case and control groups(all P values<0.01).A lowerα⁃diversity was detected in the case group than in the control group,and there was a significant difference in theβ⁃diversity between the case and control groups.At a phylum level,higher Firmicutes and Bacteroidetes abundance and lower Actinobacteria abundance were found in the case group than in the control group,and at a genus level,lower Bifidobacterium,Bacteroides and Faecalibacterium abundance and higher EnterococcusandStreptococcus abundance were seen in the case group than in the control group,while at a species level,lower B.vulgatus and B.animalis abundance and higherS.salivariusabundancewere detected in the case group than in the control group(all P values<0.05).In addition,Enterococcus,Enterobacter,Klebsiella pneumonia and Stenotrophomonas might be the specific bacteria in feces among infants infectedwith rotaviruses.Conclusion sRotavirus infection may cause remarkable alterations in gut microbiota structures among infants,suggesting that specific differential bacteria in gut microbiota may play a critical role in rotavirus

关 键 词：轮状病毒 肠道菌群 16S rRNA扩增子测序 婴幼儿 

分 类 号：R512.5[医药卫生—内科学]