Effects of Curcumin-mediated Photodynamic Therapy on Notch Signaling Pathway in a Rat Model of Cervical Cancer  

作　　者：LIU Hong-lian LIU Zhao-sheng 

机构地区：[1]Longyan First Affiliated Hospital of Fujian Medical University,Longyan Fujian Province 364000,China

出　　处：《Chinese Journal of Biomedical Engineering(English Edition)》2024年第3期122-130,共9页中国生物医学工程学报(英文版)

摘　　要：Objective: To investigate the effect of curcumin-mediated photodynamic therapy(PDT) on the Notch signaling pathway in a rat model of cervical cancer. Methods:A total of 70 SPF-grade healthy adult female Wistar rats were selected, and were randomly divided into 5 groups using a random number table: model group, positive group, curcumin group, PDT group, and PDT+curcumin group. The rat model of cervical cancer was constructed by intraperitoneal injection of U14 cells(5 ×106/mL) 1mL. After successful modeling, the model group was given intraperitoneal injection of normal saline+natural light, and the positive group was intraperitoneally injected with 100 mg/kg DAPT solution+Natural light, the curcumin group was given intraperitoneal injection of 200 μL of 150 μmol/L curcumin+natural light, the PDT group was given intraperitoneal injection of normal saline+photodynamic therapy at 100 J/cm^(2), and the PDT+curcumin group was given intraperitoneal injection of 200 μL of 150 μmol/L curcumin+photodynamic therapy at 100 J/cm^(2). Results: HE staining results showed that PDT+ curcumin group exhibited a more sparse arrangement of tumor cells in the lesion tissue slices, relatively smaller cell volume, lower nuclear-cytoplasmic ratio, and more condensed nuclei, with a noticeable presence of vesicular nuclei and perinuclear halos than the other four groups. The expression of Notch-1 protein in PDT+curcumin group, as measured by immunohistochemistry and Western blotting, was significantly lower than that in other four groups(P<0.05). CD4^(+), CD8^(+)and CD4^(+)/CD8^(+)expression levels in PDT+curcumin group were higher than those in model group, positive group and PDT group(P<0.05). The expression of Bcl-2 protein in PDT+curcumin group was lower than that in the other four groups, and the expression of Bax protein in PDT+curcumin group was higher than that in the other four groups(P<0.05). Conclusion: Curcumin-mediated PDT can inhibit Notch signaling pathway, promote immune factors, and regulate the expression of apoptosi

关 键 词：cervical cancer CURCUMIN photodynamic therapy Notch signaling pathway immune factors APOPTOSIS 

分 类 号：R-332[医药卫生] R737.33