三七药材中6种化学成分近红外定量快速检测模型建立研究  

Research on the Establishment of Near-infrared Quantitative Rapid Detection Model of 6 Chemical Components in Panax notoginseng

作　　者：王雅净 邢亚伟 张子龙[1,3] 郝巨辉[1,3] 张诗焉 鲜伊莎 李卫东 WANG Yajing;XING Yawei;ZHANG Zilong;HAO Juhui;ZHANG Shiyan;XIAN Yisha;LI Weidong(School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102488,China;Collaborative Innovation Center of Aerospace Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing 102488,China;Engineering Research Center of Ministry of Education for the Standardized Production of Chinese Herbal Medicine,Beijing 102488,China)

机构地区：[1]北京中医药大学中药学院,北京102488 [2]北京中医药大学航空航天中医药协同创新中心,北京102488 [3]中药材规范化生产教育部工程研究中心,北京102488

出　　处：《现代中药研究与实践》2024年第6期37-41,46,共6页Research and Practice on Chinese Medicines

基　　金：国家自然科学基金面上项目(82274090)

摘　　要：目的建立快速检测三七药材中6种化学成分(三七皂苷R_(1),人参皂苷Rb_(1)、Re、Rg_(1)、Rd及三七总皂苷)的模型。方法运用近红外光谱分析技术,考察粉末粒径和装样厚度对三七近红外光谱的影响,结合偏最小二乘法建立三七中三七皂苷R_(1),人参皂苷Rb_(1)、Re、Rg_(1)、Rd及三七总皂苷的定量检测模型。结果当粉末粒径≤250μm且装样厚度≥10 mm时,三七样品的近红外光谱的重复性与再现性较好;三七皂苷R_(1),人参皂苷Rb_(1)、Re、Rg_(1)、Rd及三七总皂苷定量检测模型的预测平均相对误差分别为18.01%、8.44%、14.48%、7.15%、13.46%和14.81%,预测值与真实值有较高拟合度。结论建立了三七药材中6种化学成分定量快速检测模型,为三七药材化学成分的快速定量研究提供实验依据和新思路。Objective To establish a model for the rapid detection of 6 chemical components(notoginsenoside R_(1);ginsenosides Rb_(1),Re,Rg_(1),Rd and total saponins)in Panax notoginseng.Methods Nearinfrared spectroscopy(NIR)was used to investigate the effects of powder particle size and loading thickness on the NIR spectra of P.notoginseng.Meanwhile the partial least squares method was combined to establish a quantitative detection model for notoginsenoside R_(1);ginsenosides Rb_(1),Re,Rg_(1),Rd and total saponins in P.notoginseng.Results The repeatability and reproducibility of the near-infrared spectra of P.notoginseng samples were good when the powder particle size≤250μm and the loading thickness≥10 mm.The average relative errors of the predictions of the quantitative detection models for notoginsenoside R_(1);ginsenosides Rb_(1),Re,Rg_(1),Rd and total saponins were 18.01%,8.44%,14.48%,7.15%,13.46%and 14.81%.The predicted values had a high degree of fit with the true values.Conclusion The models for the quantitative and rapid detection of 6 chemical components in P.notoginseng are established to provide experimental basis and new ideas for the rapid quantitative study of the chemical components in P.notoginseng.

关 键 词：三七 三七皂苷 人参皂苷 近红外光谱 定量模型 

分 类 号：R285.5[医药卫生—中药学]