基于线粒体Cytb的新疆黑条小车蝗种群遗传多样性及谱系地理学研究  

Genetic diversity and phylogeography of Oedaleus decorus decorus(Orthoptera:Oedipodidae)populations in Xinjiang,western China based on the mitochondrial Cytb

作　　者：马钰洁 张晓清 王一鸣 钟圆佳 季荣[1,2,3] 袁亮 MA Yu-Jie;ZHANG Xiao-Qing;WANG Yi-Ming;ZHONG Yuan-Jia;JI Rong;YUAN Liang(International Center for the Collaborative Management of Cross-border Pests in Central Asia,School of Life Sciences,Xinjiang Normal University,Urumqi 830054,China;Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology,School of Life Sciences,Xinjiang Normal University,Urumqi 830054,China;Key Laboratory of Special Environment Biodiversity Application and Regulation in Xinjiang,School of Life Sciences,Xinjiang Normal University,Urumqi 830054,China)

机构地区：[1]新疆师范大学生命科学学院,中亚区域跨境有害生物联合控制国际联合研究中心,乌鲁木齐830054 [2]新疆师范大学生命科学学院,新疆特殊环境物种保护与调控生物学实验室,乌鲁木齐830054 [3]新疆师范大学生命科学学院,新疆特殊环境物种多样性应用与调控重点实验室,乌鲁木齐830054

出　　处：《昆虫学报》2025年第1期98-109,共12页Acta Entomologica Sinica

基　　金：2024年新疆维吾尔自治区中央引导地方科技发展专项资金项目(ZYYD2024ZY01);国家自然科学基金项目(32260254)。

摘　　要：【目的】本研究旨在揭示中国西部新疆地区黑条小车蝗Oedaleus decorus decorus不同地理种群的遗传多样性及谱系关系,为新疆迁飞蝗虫的历史迁飞规律提供分子遗传学证据。【方法】PCR扩增新疆28个黑条小车蝗地理种群线粒体Cytb上740 bp的核酸序列,基于Cytb片段序列利用DnaSP 5.10和Arlequin 3.1软件对黑条小车蝗地理种群的遗传多样性、遗传分化格局和基因流等进行分析。【结果】从新疆28个黑条小车蝗地理种群的338条Cytb片段中共检出27个单倍型,群体单倍型多样性指数(Hd)为0.8185,核苷酸多样性(π)为0.00611,暗示黑条小车蝗种群遗传多样性水平较高;基于单倍型构建的分子系统发育树及median-joining网络关系分析表明,新疆地区黑条小车蝗地理种群被明显划分为2个地理遗传谱系:天山北麓谱系(谱系Ⅰ,包括温泉、沙湾、昌吉、乌鲁木齐和哈密种群及博乐种群的1头个体)和中哈边境谱系(谱系Ⅱ,包括伊犁、塔城和阿勒泰种群及博乐种群的11头个体);AMOVA分析显示新疆黑条小车蝗不同地理种群间的遗传变异较高(71.38%),而不同地理种群内的遗传变异水平较低(28.62%);28个地理种群间的遗传分化指数(Fst)为-0.097~0.987,2个地理遗传谱系之间遗传分化指数(Fst)为0.805;核苷酸错配分布呈现多峰,Tajima’s D值为0.193,Fu’s Fs为-2.863,推测新疆地区黑条小车蝗未发生历史种群扩张。【结论】新疆地区黑条小车蝗具有丰富的遗传多样性和明显的系统地理分布格局;2个地理谱系间基因交流较弱,存在较大遗传分化,但2个地理谱系内各种群存在较强基因流,推测是高山、沙漠等地理隔离所致。该研究结果为重构新疆草原蝗虫历史迁飞路径及阐明当前分布格局提供分子遗传学依据。【Aim】The aim of this study is to reveal the genetic diversity and genealogical relationships of different geographic populations of Oedaleus decorus decorus in Xinjiang,western China,and to provide molecular genetic evidence for the historical migratory patterns of migratory locusts in Xinjiang.【Methods】PCR was used to amplify the 740 bp of nucleic acid sequence on the mitochondrial Cytb of 28 geographic populations of O.decorus decorus in Xinjiang.The genetic diversity,patterns of genetic differentiation and gene flow in geographic populations of O.decorus decorus were analyzed based on Cytb fragment sequences using DnaSP 5.10 and Arlequin 3.1 software.【Results】A total of 27 haplotypes were detected in 338 Cytb fragments from 28 geographic populations of O.decorus decorus in Xinjiang,and the population haplotype diversity index(Hd)was 0.8185 and the nucleotide diversity(π)was 0.00611,implying a high level of population genetic diversity of O.decorus decorus populations.Molecular phylogenetic trees constructed based on haplotypes and median-joining network relationship analyses showed that the geographic populations of O.decorus decorus in Xinjiang were clearly divided into two geographic genetic lineages:The northern Tianshan foothills lineage(lineage I,including Wenquan,Shawan,Changji,Urumqi and Hami populations,and 1 individual from the Bole population)and the Sino-Harbin border lineage(lineage II,including Ili,Tacheng and Altay populations,and 11 individuals from the Bole population).AMOVA analysis showed high genetic variation(71.38%)among different geographic populations of O.decorus decorus in Xinjiang,while the level of genetic variation within different geographic populations was low(28.62%).The genetic differentiation index(Fst)among the 28 geographic populations ranged from-0.097 to 0.987,and that between the two geographic genetic lineages was 0.805.The distribution of nucleotide mismatches showed multiple peaks,with the Tajima’s D value of 0.193 and Fu’s Fs value of-2.863,hypothesizi

关 键 词：黑条小车蝗 CYTB 遗传多样性 谱系地理学 基因流 

分 类 号：Q963[生物学—昆虫学]