盐碱胁迫下莠去津对高粱氧化应激及解毒酶活性的影响  

Effect of atrazine on oxidative stress and detoxification enzyme activities in Sorghum bicolor(L.)Moench under saline-alkaline stress

作　　者：汪芷璇 郭浩铭 翟王晶 梁亚博 刘雪科 王鹏[1] 周志强[1] 刘东晖[1] WANG Zhixuan;GUO Haoming;ZHAI Wangjing;LIANG Yabo;LIU Xueke;WANG Peng;ZHOU Zhiqiang;LIU Donghui(Department of Applied Chemistry,College of Science,China Agricultural University,Beijing 100193,China)

机构地区：[1]中国农业大学理学院应用化学系,北京100193

出　　处：《农药学学报》2025年第1期80-89,共10页Chinese Journal of Pesticide Science

基　　金：国家重点研发计划课题(No.2023YFD1701305).

摘　　要：盐碱土中农药的环境行为会发生变化,并可能会对作物产生潜在风险,然而目前有关盐碱地施用农药后的风险评估数据不足。本研究通过室内盆栽试验,以耐盐碱作物高粱为研究对象,探究了盐碱胁迫下施用莠去津在高粱体内的残留分布和毒性风险。结果表明:盐碱胁迫下莠去津在土壤中的半衰期延长了38.33%~105.94%;空白土壤中施用莠去津,对高粱株高、根部氧化应激相关酶和解毒酶均无显著影响。在质量分数为0.2%的中性盐和碱性盐胁迫下,高粱根部氧化应激标志物活性氧(ROS)含量分别上升了37.91%和28.67%,丙二醛(MDA)含量分别上升了5.26%和40.27%,表明在中度盐碱胁迫下施用莠去津会对高粱造成氧化损伤。在0.2%中性盐胁迫下,施用莠去津使得高粱根部细胞色素P450酶(CYP450)和谷胱甘肽S-转移酶(GSTs)的活性分别下降了41.56%和29.76%,说明在中度盐胁迫下施用莠去津抑制了高粱的代谢解毒过程。本研究可为盐碱地中莠去津的施用风险提供参考,关注实际农业生产场景下农药的环境风险,可为盐碱地科学精准施药提供理论依据。The environmental behavior of pesticides could be altered in saline-alkaline soils,which might pose a potential risk to crops.Research on regarding the risk assessment of pesticide application in saline-alkaline soils was still limited.In this study,the accumulation of atrazine and toxicity risk of atrazine in salt-tolerant Sorghum bicolor(L.)Moench under saline-alkaline stress using indoor pot experiments were explored.The results revealed that atrazine exhibited prolonged persistence by 38.33%to 105.94%in soil under saline-alkaline stress.Atrazine had no significant effect on sorghum plant height,root oxidative stress enzymes and detoxification enzymes in control soil.Under 0.2%neutral and alkaline salt stress,markers of oxidative stress such as reactive oxygen species(ROS)content increased by 37.91%and 28.67%,and malondialdehyde(MDA)increased by 5.26%and 40.27%,respectively.The increase of ROS and MDA content suggested that atrazine caused oxidative damage to sorghum under moderate saline-alkaline stress.Under 0.2%neutral salinity stress,application of atrazine decreased the activities of cytochrome P450 enzymes(CYP450)and glutathione S-transferases(GSTs)in sorghum roots by 41.56%and 29.76%,respectively,indicating that salinealkaline stress inhibited metabolic detoxification processes.This study provides a reference for the application risk of atrazine in saline-alkaline soil,focuses on the environmental risk of pesticides in actual agricultural production scenarios,and provides a theoretical basis for scientific and precise application of pesticides in saline-alkaline soils.

关 键 词：莠去津 盐碱胁迫 高粱 作物毒性 氧化应激 解毒酶 

分 类 号：S481.1[农业科学—农药学] X131.1[农业科学—植物保护]