甲氨基阿维菌素苯甲酸盐在马尾松树体内的代谢产物研究  

Study on metabolism degradation of emamectin benzoate in Pinus massoniana

作　　者：姜壮 宋林松 李云帆 蔡伟飞 初楚 陈安良[3] 王继栋 张绍勇 JIANG Zhuang;SONG Linsong;LI Yunfan;CAI Weifei;CHU Chu;CHEN Anliang;WANG Jidong;ZHANG Shaoyong(Meitan Forestry Bureau of Guizhou Province,Zunyi 564100,Guizhou Province,China;Key Laboratory of Vector Biology and Pathogen Transmission Control in Zhejiang Province,Huzhou University,Huzhou 313000,Zhejiang Province,China;National Local Joint Engineering Laboratory of High efficiency Preparation Technology of Biopesticide,Zhejiang Agriculture and Forestry University,Hangzhou 311300,China;Forest Songyang Resources Administration of Zhejiang Province,Lishui 323400,Zhejiang Province,China)

机构地区：[1]贵州省湄潭县林业局,贵州遵义564100 [2]湖州师范学院浙江省媒介生物学与病原传播控制重点实验室,浙江湖州313000 [3]浙江农林大学生物农药高效制备技术国家地方联合工程实验室,杭州311300 [4]浙江省松阳县森林资源保护管理站,浙江丽水323400

出　　处：《农药学学报》2025年第1期107-115,共9页Chinese Journal of Pesticide Science

基　　金：浙江省重点研发计划项目(No.2019C02024);湖州市公益性研究项目(No.2021GZ25);教育厅科研项目(No.Y202043997);国家大创项目(No.202210347005).

摘　　要：为了明确甲氨基阿维菌素苯甲酸盐(以下简称甲维盐)注干施药后的代谢产物和降解途径,研究了甲维盐在马尾松树体内的代谢途径、代谢产物及其活性。通过超声浸提、硅胶柱层析、凝胶柱层析、制备色谱、高效液相色谱(HPLC)、液-质联用色谱(LC-MS)、室内生测7个步骤,对甲维盐注干施药17个月后的马尾松树体内进行残留分析和代谢产物鉴定。结果表明:甲维盐保留率为50.05%~54.26%。在马尾松树体内分离到16种甲维盐代谢产物,表征了其中11种代谢产物,分别为C_(49)H_(77)NO_(14)、C_(49)H_(75)NO_(14)、C_(41)H_(60)O_(11)(MSB_(1a))、C_(42)H_(63)NO_(10)、C_(49)H_(77)NO_(14)、C_(49)H_(73)NO_(14)(FAB_(1a))、C_(48)H_(72)O_(14)(4″-S-OH-B_(1a))、C_(49)H_(75)NO_(13)、C_(50)H_(77)NO_(13)、C_(50)H_(77)NO_(13)和C_(49)H_(77)NO_(13)。10 mg/L代谢产物混合物处理松材线虫Bursaphelenchus xylophilus 24 h的死亡率为69.12%。在树体内无光条件下,甲维盐分解代谢速度较慢,分解的代谢产物对松材线虫也具有杀虫活性。本研究解释了甲维盐注干施药可以在较长时间内有效防治松材线虫病的原因。To clarify the metabolites and degradation pathways of emamectin benzoate(EB)after trunk injection,the metabolic degradation,metabolites,and their activities of EB in Pinus massoniana were investigated.Residue analysis and metabolite identification were carried out using ultrasonic extraction,silica gel column chromatography,gel column chromatography,preparative chromatography,HPLC,LC-MS,and indoor bioactivity test in P.massoniana after 17 months of trunk application of EB.The retention rate of EB was 50.05%-54.26%.Sixteen metabolites of EB in P.massoniana were isolated and 11 of them were characterized by their molecular formulas:C_(49)H_(77)NO_(14),C_(49)H_(75)NO_(14),C_(41)H_(60)O_(11)(MSB_(1a)),C_(42)H_(63)NO_(10),C_(49)H_(77)NO_(14),C_(49)H_(73)NO_(14)(FAB_(1a)),C_(48)H_(72)O_(14)(4″-S-OH-B_(1a)),C_(49)H_(75)NO_(13),C_(50)H_(77)NO_(13),C_(50)H_(77)NO_(13) and C_(49)H_(77)NO_(13).The mortality of Bursaphelenchus xylophilus exposed to the metabolite mixture at 10 mg/L for 24 hours was 69.12%.In the absence of light inside the tree,the metabolism of EB is slower and the catabolic products remain active against pine wood nematodes.This study explains why the dry application of EB can be effective in controlling pine wood nematode disease over a longer period.

关 键 词：甲氨基阿维菌素苯甲酸盐 马尾松树 注干施药 代谢产物 生物活性 

分 类 号：S482[农业科学—农药学] S763.7[农业科学—植物保护]