藤仓镰刀菌对氰烯菌酯的抗性及其治理  

Resistance management of Fusarium fujikuroi to phenamacril

作　　者：董代幸 葛晨阳 董怡 毛程鑫 张传清[1] DONG Daixing;GE Chenyang;DONG Yi;MAO Chengxin;ZHANG Chuanqing(College of Advanced Agricultural Sciences,Zhejiang A&F University,Hangzhou 311300,China;Fuyang District Agricultural Technology Extension Center of Hangzhou City,Zhejiang Province,Hangzhou 311400,China)

机构地区：[1]浙江农林大学现代农学院,杭州311300 [2]浙江省杭州市富阳区农业技术推广中心,杭州311400

出　　处：《农药学学报》2025年第1期153-159,共7页Chinese Journal of Pesticide Science

基　　金：国家自然科学基金项目(32472596).

摘　　要：为明确浙江省水稻恶苗病致病菌藤仓镰刀菌对氰烯菌酯的抗性及其治理的替换药剂方案,于2021—2022年从浙江杭州和金华市共分离获得196株藤仓镰刀菌,分析了其对氰烯菌酯的抗性频率、抗性水平和抗性机制,进一步通过菌丝生长速率法、盆栽法及田间防效试验综合评估了氯氟醚菌唑用于治理藤仓镰刀菌氰烯菌酯抗性菌株的可行性。结果表明:2021年,从杭州、金华两地分离的86株藤仓镰刀菌菌株中,有10株为氰烯菌酯抗性菌株,抗性频率为11.63%;2022年,从两地分离的110株菌株中,有22株为氰烯菌酯抗性菌株,抗性频率为20.00%,与2021年比呈上升趋势。藤仓镰刀菌氰烯菌酯抗性菌株的抗性水平范围为145.963~235.256,平均为185.379,均为高水平抗性,抗性菌株的Myosin-5基因均发生K218T点突变。氯氟醚菌唑对藤仓镰刀菌的EC50值在0.108~0.342μg/mL之间,平均EC50值为0.221μg/mL,在氰烯菌酯抗性和敏感菌株的EC50值之间无显著差异。在盆栽试验中,10μg/mL氯氟醚菌唑能够显著抑制藤仓镰刀菌对氰烯菌酯敏感及抗性菌株导致的水稻幼苗徒长。在田间试验中,400 g/L氯氟醚菌唑悬浮剂5000倍液种子处理对水稻恶苗病也表现出优异的防效。本研究结果表明,氯氟醚菌唑可以作为氰烯菌酯的替换药剂有效防控水稻恶苗病,延缓藤仓镰刀菌对氰烯菌酯的抗性。In order to determine the resistance of Fusarium fujikuroi to phenamacril in Zhejiang Province and the alternative agent for its control,this study analyzed the resistance frequency,resistance level and resistance mechanism of 196 F.fujikuroi isolates collected from Hangzhou City and Jinhua City in Zhejiang Province,and also evaluated the feasibility of mefentrifluconazole against phenamacril-resistant F.fujikuroi strains via mycelium growth rate method,pot method and field efficacy test.The results showed that in 2021,10 out of 86 isolates of F.fujikuroi isolated from Hangzhou City and Jinhua City were phenamacril-resistant isolates,with a resistance frequency was 11.63%;in 2022,22 out of 110 isolates of F.fujikuroi isolated from Hangzhou City and Jinhua City were phenamacril-resistant isolates,and the resistance frequency was 20.00%.The resistance levels of the resistant F.fujikuroi isolates ranged from 145.963-235.256,and the average resistance level was 185.379,indicating a high-level resistance.The corresponding resistance mechanism was caused by K218T mutation of Myosin-5.The EC50 values of the phenamacril-resistant and-sensitive strains to mefentrifluconazole ranged from 0.108 to 0.342μg/mL,and the average EC50 value were 0.221μg/mL.In the pot experiments,the application of mefentrifluconazole 10μg/mL can significantly inhibit the excessive growth of rice caused by phenamacril-resistant strains.Additionally,in the field assay,mefentrifluconazole 400 g/L SC 5000-fold dilution also showed an excellent control efficacy on rice bakanae disease.The results of this study indicated that mefentrifluconazole could be used as an alternative agent for the prevention and control of rice bakanae disease and delay the resistance of F.fujikuroi to phenamacril.

关 键 词：水稻恶苗病 藤仓镰刀菌 氰烯菌酯 氯氟醚菌唑 抗性治理 

分 类 号：S435.111.44[农业科学—农业昆虫与害虫防治]