阴道超声联合血清SFRP4、CYP19A1检测诊断PCOS效能  

Efficacy of the ultrasound examination combined with the serum secreted frizzled related protein 4(SFRP4)and cytochrome P45019A1 levels of women for diagnosing their polycystic ovary syndrome

作　　者：洪佩娟 张琛琛 许丽琴 杨桂红 HONG Peijuan;ZHANG Chenchen;XU liqin;YANG Guihong(Linhai Maternity and Child Health Care Hospital,Linhai,Zhejiang Province,317000;Taizhou Enze Medical Group(Center)Taizhou Hospital,Taizhou,Zhejiang Province)

机构地区：[1]浙江临海市妇幼保健院,317000 [2]浙江台州恩泽医疗集团(中心)台州医院

出　　处：《中国计划生育学杂志》2025年第2期397-403,共7页Chinese Journal of Family Planning

摘　　要：目的:探讨阴道超声联合血清分泌型卷曲相关蛋白4(SFRP4)、细胞色素P450 19A1(CYP19A1)检测在多囊卵巢综合征(PCOS)诊断中的应用价值。方法:选取2022年7月-2024年8月在本院诊治的PCOS患者106例为PCOS组,体检健康女性106例为对照组,两组均行阴道超声检查。实时荧光定量PCR(qRT-PCR)仪检测血清SFRP4、CYP19A1mRNA表达;Pearson法分析超声参数与血清SFRP4,CYP19A1表达水平的相关性,多因素logistic回归分析PCOS的影响因素;受试者工作特征(ROC)曲线分析超声参数联合血清SFRP4、CYP19A1mRNA水平对PCOS的诊断价值。结果:PCOS组OV、TA、SA、FN、EDV、PSV、血清SFRP4 mRNA(2.58±0.51)水平均大于对照组(1.01±0.24),PI、RI、血清CYP19A1 mRNA水平(0.71±0.11)均小于对照组(1.00±0.22)(均P<0.05)。血清SFRP4表达水平与OV、TA、SA、FN、EDV、PSV均呈正相关,与PI、RI呈负相关;血清CYP19A1表达水平与OV、TA、SA、FN、EDV、PSV均呈负相关,与PI、RI呈正相关,SFRP4 mRNA升高是影响PCOS发生的危险因素,CYP19A1 mRNA升高是PCOS发生保护因素(均P<0.05)。ROC分析,超声参数联合血清SFRP4、CYP19A1 mRNA诊断PCOS的曲线下面积(0.987)最大,优于各指标单独诊断(均P<0.05)。结论:PCOS患者OV、TA、SA、FN、EDV、PSV以及血清SFRP4 mRNA水平升高,PI、RI以及血清CYP19A1 mRNA水平下降,各指标具有相关性,超声参数联合血清SFRP4、CYP19A1检测能够提高对PCOS的诊断价值。Objective: To explore the value of transvaginal the ultrasound examination combined with the serum secreted frizzled related protein 4(SFRP4) and cytochrome P450 19A1(CYP19A1) levels of women for diagnosing their polycystic ovary syndrome(PCOS). Methods: A total of 106 women with PCOS who underwent diagnosis and treatment in the hospital were selected in study group and another 106 healthy women who underwent physical examination in the hospital were selected in control group from July 2022 to August 2024. The women in both groups were given the transvaginal ultrasound examination. Real-time fluorescence quantitative PCR(qRT-PCR) was used to detect the serum SFRP4 and CYP19A1 mRNA expressions of the women in the two groups. Pearson method was used to analyze the correlation between the ultrasound parameters of the women and their expression levels of serum SFRP4 and CYP19A1. Multivariate logistic regression was applied to analyze the influencing factors of the PCOS occurrence of the women. Receiver operating characteristic(ROC) curve was applied to analyze the diagnostic value of the ultrasound parameters combined with the serum SFRP4 and CYP19A1 mRNA levels of the women for their PCOS occurrence. Results: The values of OV, TA, SA, FN, EDV and PSV of the women in the study group were significantly higher than those of the women in the control group.The serum SFRP4 mRNA level(2.58±0.51)of the women in the study group was significantly higher than that(1.01±0.24)of the women in the control group.The values of PI and RI of the women in the study group were significantly lower than those of the women in the control group.The serum CYP19A1mRNA level(0.71±0.11)of the women in the study group was significantly lower than that(1.00±0.22)of the women in the control group(all P<0.05).The expression level of the serum SFRP4of the women in the study group was positively correlated with their OV,TA,SA,FN,EDV and PSV values,and was negatively correlated with their PI and RI values.The expression level of the serum CYP19A1o

关 键 词：多囊卵巢综合征 阴道超声 分泌型卷曲相关蛋白4 细胞色素P45019A1 相关性 诊断 

分 类 号：R711.75[医药卫生—妇产科学]