牛奶中恩诺沙星抗生素残留的SERS检测研究  

SERS Detection of Enrofloxacin Antibiotic Residues in Milk

作　　者：张仲鑫 王佳雯 王晨旭 秦苗 ZHANG Zhongxin;WANG Jiawen;WANG Chenxu;QIN Miao(School of Chemistry and Chemical Engineering,Suzhou University,Suzhou 234000,China)

机构地区：[1]宿州学院化学化工学院,安徽宿州234000

出　　处：《山东化工》2025年第2期169-172,179,共5页Shandong Chemical Industry

基　　金：宿州学院资助2024年国家级大学生创新创业训练计划项目,项目名称:纳米腔SERS空间热点对偶氨化合物光致异构过程的原位动态监测研究;宿州学院2024年博士(后)科研启动基金项目(2024BSK011);安徽省中青年教师培养行动优秀青年教师培育项目(YQYB2023050);2022年度安徽省质量工程“四新”研究与改革实践项目“新工科”背景下地方性应用型高校应用化学专业实践教学改革探索与实践(2022sx150)。

摘　　要：恩诺沙星作为氟喹诺酮类抗生素,在食品医学等方面被广泛应用,过量使用抗生素会对人体健康产生很大的危害。在本文中,使用表面增强拉曼光谱(Surface-enhanced Raman spectroscopy,SERS)作为分析基础,并使用晶种生长法来制备金纳米立方体,用来作为SERS检测基底,采用结晶紫(crystal violet,CV)作为SERS增强的探针分子,检测牛奶中氟喹诺酮类抗生素残留。运用紫外-可见光谱仪(UV-Vis)和扫描电子显微镜(SEM)对金纳米立方体进行形貌表征。最终使用标准加入法测定真实牛奶样品中恩诺沙星。结果表明,恩诺沙星在乙酸乙酯标准溶液中的检测限为10^(-6)mol/L,在牛奶中恩诺沙星检测限为10^(-4)mol/L。实验结果为牛奶等液体食品安全检测提供一定研究意义。Enrofloxacin,as a fluoroquinolone antibiotic,is widely used in food medicine and other fields.Overuse of antibiotics poses great harm to human health.In this article,Surface enhanced Raman spectroscopy(SERS)was used as the analytical basis,and gold nanocubes were prepared using seed growth method as the SERS detection substrate.Crystal violet(CV)was used as the SERS enhanced probe molecule to detect residual fluoroquinolone antibiotics in milk.The morphology of gold nanocubes was characterized using UV-Vis and SEM.Finally,the standard addition method was used to determine enrofloxacin in real milk samples.The results showed that the detection limit of enrofloxacin in ethyl acetate standard solution was 10^(-6) mol/L,and the detection limit of enrofloxacin in milk was 10^(-4) mol/L.The experimental results provide certain research significance for the safety testing of liquid foods such as milk.

关 键 词：金纳米立方体 牛奶 恩诺沙星 表面增强拉曼光谱 

分 类 号：O657.37[理学—分析化学]