基于Nirf2/keap1信号通路人参皂苷Ck对认知障碍小鼠神经保护作用的机制研究  

Mechanisms of Neuroprotective Effects of Ginsenoside CK on Cognitively Impaired Mice Based on Nrf2/Keap1 Signaling Pathway

作　　者：杨擎[1] 张芮 李娜[1] 李辉[3] 曹占鸿 房星宇 李典育 安昱 白浩楠 刘健[1] 陈世范 YANG Qing;ZHANG Rui;LI Na;LI Hui;CAO Zhanhong;FANG Xingyu;LI Dianyu;AN Yu;BAI Haonan;LIU Jian;CHEN Shifan(Changchun University of Chinese Medicine,Changchun 130117,China;The Second Norman Bethune Hospital of Jilin University,Changchun 130041,China;Jilin Qianwei Hospital,Changchun 130012,China)

机构地区：[1]长春中医药大学,吉林长春130117 [2]吉林大学白求恩第二医院,吉林长春130041 [3]吉林省前卫医院,吉林长春130012

出　　处：《特产研究》2025年第1期103-108,共6页Special Wild Economic Animal and Plant Research

基　　金：吉林省科技发展计划项目(YDZJ202301ZYTS466);吉林省教育厅科学研究项目(JJKH20220884KJ、JJKH20210989KJ),吉林省大学生创新训练计划项目(S202110199093)。

摘　　要：本研究旨在明确人参皂苷CK通过对Nrf2/Keap1通路基因的调控,改善小鼠认知障碍和神经元保护作用的分子机制研究。本研究通过腹腔注射氢溴酸东莨菪碱(SCOP)创建小鼠认知障碍模型,通过水迷宫系统检测各组小鼠的记忆和学习能力,通过尼氏染色观察脑组织神经元尼氏体数量和形态,采用试剂盒检测血清中GSH含量,利用实时荧光定量PCR检测Nrf2/Keap1信号通路和凋亡相关基因的表达水平。结果表明,人参皂苷CK提高认知障碍小鼠学习记忆能力,抑制小鼠脑组织海马神经元损伤,提高血清内GSH含量,调节脑内Nrf2/Keap1信号通路相关基因的表达,抑制脑组织细胞凋亡。人参皂苷CK可通过调控Nrf2/Keap1信号通路相关基因,抑制小鼠脑组织神经元氧化损伤和凋亡,改善小鼠认知障碍。To clarify the molecular mechanism by which ginsenoside CK ameliorates cognitive impairment and neuronal protection in mice through the regulation of Nrf2/Keap1 pathway genes.In this study,a mouse model of cognitive impairment was established by intraperitoneal injection of scopolamine hydrobromide(SCOP).Memory and learning capacity of mice tested by water maze experiment,number of neuronal nissl body and neuronal morphology in brain tissue as observed by nissl staining,and the concentration of GSH in the serum of mice was measured by a kit.Expression of genes related to the Nrf2/Keap1 signalling pathway and apoptotic pathways were examined using realtime fluorescence quantitative PCR.Ginsenoside CK improves the learning and memory ability of cognitively impaired mice,inhibits hippocampal neuronal damage in mouse brain tissue,increases the level of GSH in serum,regulates the expression of genes related to Nrf2/Keap1 signaling pathway in brain,and inhibits apoptosis in brain tissue.Ginsenoside CK can inhibit oxidative damage and apoptosis of neurons in mouse brain tissue and improve cognitive impairment in mice by regulating genes related to the Nrf2/Keap1 signaling pathway.

关 键 词：人参皂苷CK Nrf2/Keap1信号通路 认知障碍 神经保护作用 

分 类 号：R285.5[医药卫生—中药学]