基于重组酶介导等温核酸扩增技术快速检测食品中骆驼源性成分的方法建立及评价  

Establishing and evaluating a rapid detection method for camel-derived ingredients in food based on recombinase polymerase amplification technology

作　　者：周臣清 张娟 綦艳 赵玲 黄宝莹 杨纯佳 ZHOU Chenqing;ZHANG Juan;QI Yan;ZHAO Ling;HUANG Baoying;YANG Chunjia(Guangdong Testing Institute of Product Quality Supervision,Guangdong Engineering Technology Research Center for Food Biohazard Monitoring,Foshan 528300,China)

机构地区：[1]广东产品质量监督检验研究院,广东省食品生物危害因素监测工程技术研究中心,广东佛山528300

出　　处：《中国乳品工业》2025年第2期75-80,共6页China Dairy Industry

基　　金：国家市场监督管理总局科技项目(2024MK100);广东省市场监督管理局项目(2022CZ03);广东产品质量监督检验研究院内部项目(GQI202304)。

摘　　要：文章开发一种基于重组酶介导等温核酸扩增技术(Recombinase Aided Amplification,RAA)的方法,实现对食品中骆驼源性成分快速检测。针对骆驼细胞色素B(Cytochrome b,Cyt b)基因设计特异性引物,对建立的RAA体系优化其反应扩增条件,评估其特异性和灵敏度,并用建立的RAA法与GB/T 38164标准方法分别对24份市售骆驼制品进行检测并比对结果。结果表明,本研究建立的RAA法特异性强,乳及乳制品检出限为1.0%,肉及肉制品检出限为0.1%,且2种方法对24份骆驼乳肉及其制品检测结果一致。因此,本研究开发的RAA方法具有快速、特异性强、灵敏度高、操作简单等优点,市场应用前景广阔。Developing a method based on Recombinase Aided Amplification(RAA)for the rapid detection of camel-derived ingredients in food.Specific primers were designed targeting the Cytochrome b(Cyt b)gene of camels,and the RAA system's reaction conditions were optimized.The specificity and sensitivity of the established RAA method were evaluated.Twenty-four commercially available camel products were tested using both the established RAA method and the GB/T 38164 standard method,and the results were compared.The outcomes revealed that the developed RAA method exhibited strong specificity,with detection limits of 0.1%for meat and meat products and 1.0%for milk and dairy products.The results from both methods were consistent for all 24 camel products.Therefore,the RAA method developed in this study possesses advantages such as rapidity,strong specificity,high sensitivity,and operational simplicity,suggesting broad applications in the field.

关 键 词：重组酶介导等温核酸扩增技术 骆驼源性成分 细胞色素B基因 快速检测 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]