代谢组学视角研究水飞蓟宾对缓解小鼠非酒精性脂肪肝的作用  

Study on the effect of silibinin in alleviating non-alcoholic fatty liver disease in mice from the perspective of metabonomics

作　　者：刘青 贺佳诺 赵凌云 赵山山 郝光飞 刘洪伟[2] LIU Qing;HE Jianuo;ZHAO Lingyun;ZHAO Shanshan;HAO Guangfei;LIU Hongwei(School of Life Science and Food Engineering,Hebei University of Engineering,Handan Hebei 056038,China;Institute of Biology,Hebei Academy of Sciences,Shijiazhuang Hebei 050081,China)

机构地区：[1]河北工程大学生命科学与食品工程学院,河北邯郸056038 [2]河北省科学院生物研究所,河北石家庄050081

出　　处：《河北省科学院学报》2025年第1期52-60,共9页Journal of The Hebei Academy of Sciences

基　　金：河北省重点研发计划项目(23372401D);河北省科学院科技计划项目(24308);河北省科学院高层次人才培养与资助项目(2024G11);新神农百草计划项目(2024SN01)。

摘　　要：本文采用高脂粮饲喂模型小鼠8周,随机分为对照组、低剂量水飞蓟宾灌胃组(37.8 mg/kg)、高剂量水飞蓟宾灌胃组(75.6 mg/kg),对照组灌胃等量生理盐水。灌胃28 d后,取出肝脏,采用液相色谱质谱法(LC-MS)进行代谢组学分析。结果显示,高低水飞蓟宾灌胃组均会使肝脏内乙酰辅酶A、L-谷氨酸、谷胱甘肽、谷胱甘肽二硫化物、胞苷二磷酸乙醇胺、sn-甘油-3-磷酸、磷脂酰胆碱、乙酰胆碱等有利于缓解小鼠非酒精性脂肪肝的物质表达量上升。In this study,model mice were fed a high-fat diet for 8 weeks and randomly assigned to a control group,a low-dose silibinin gavage group(37.8 mg/kg),and a high-dose silibinin gavage group(75.6 mg/kg).The control group received an equivalent volume of saline.After 28 days of gavage,the liver was harvested,and metabolomic analysis was conducted using liquid chromatography-mass spectrometry(LC-MS).The results showed that intrahepatic expression of metabolites conducive to alleviating non-alcoholic fatty liver disease(NAFLD)in mice,including acetyl-CoA,L-glutamate,glutathione,glutathione disulfid,CDP-ethanolamine,sn-glycerol-3-phosphate,choline phosphate,acetylcholine was significantly upregulated in both the low-dose and high-dose silibinin gavage groups.

关 键 词：水飞蓟宾 代谢组学 肝脏 差异代谢物 差异代谢通路 

分 类 号：R285.5[医药卫生—中药学]