骆驼痘病毒实时荧光PCR检测方法的建立  

Establishment of the Real-time Fluorescent PCR Method for Camelpox Virus

作　　者：朱道中 梅明珠 李明 佟铁铸 王莹 丁宁 徐娟 王春霞 ZHU Daozhong;MEI Mingzhu;LI Ming;TONG Tiezhu;WANG Ying;DING Ning;XU Juan;WANG Chunxia(Guangzhou Customs Technology Center,Guangzhou Guangdong 510623)

机构地区：[1]广州海关技术中心,广东广州510623

出　　处：《广东畜牧兽医科技》2025年第1期46-51,共6页Guangdong Journal of Animal and Veterinary Science

基　　金：重要动物疫病跨境传播风险防控关键技术及示范应用(2023B04J0137)。

摘　　要：骆驼痘(Camelpox,CML)是一种广泛流行的骆驼病毒性传染病,它主要发生于中东和东北非的大规模骆驼群,给骆驼产业造成了巨大的经济损失。该研究通过对GenBank中所公布的13个骆驼痘病毒分离株全基因组核苷酸序列进行同源性比较,选取其ATIP基因保守序列,筛选出了一套特异性引物和探针,建立了相应的实时荧光PCR检测方法。结果表明,该方法特异性强、重复性好、灵敏度高,其最低检测限度为7.8 copies/μL。Camelpox(CML)is a widespread infectious viral disease of camels that occurs mostly in the Middle East and in North East Africa on a substantial scale and causes huge economic losses to the camel industry.In the present study,the homology comparison of the complete genome nucleotide sequences of 13 camelpox virus isolates published in GenBank were performed,then a set of specific primers and a specific probe based on their conserved sequences of the ATIP gene were designed and a real-time fluorescent polymerase chain reaction(PCR)assay was developed.The results showed that this method had high specificity,good repeatability,high sensitivity,and its minimum detectable limit was 7.8 copies/μL.

关 键 词：骆驼痘病毒 实时荧光PCR 方法建立 

分 类 号：S852.65[农业科学—基础兽医学]