广东某猪场母猪流产的病原检测和分析  

Pathogen Detection and Analysis of Sow Abortions in a Pig Farm in Guangdong

作　　者：聂晶晶 陈家璐 陈家玥 徐绍栋 瞿云芝 刘志成 张建峰 张春红 NIE Jingjing;CHEN Jialu;CHEN Jiayue;XU Shaodong;QU Yunzhi;LIU Zhicheng;ZHANG Jianfeng;ZHANG Chunhong(Key Laboratory of Livestock Disease Prevention of Guangdong Province,Institute of Animal Health,Guangdong Academy of Agricultural Sciences,Guangzhou Guangdong 510640;Guangzhou Dafu Breeding Co.,Ltd.,Guangzhou Guangdong 510830)

机构地区：[1]广东省农业科学院动物卫生研究所/广东省畜禽疫病防治研究重点实验室,广东广州510640 [2]广州市大福养殖有限公司,广东广州510830

出　　处：《广东畜牧兽医科技》2025年第1期110-117,共8页Guangdong Journal of Animal and Veterinary Science

基　　金：广州市科技计划项目(No.2024E04J0466);广东省畜禽疫病防治研究重点实验室项目(No.2023B1212060040);猪禽种业全国重点实验室项目(No.2023QZ-NK13,ZQQZ-55)。

摘　　要：2024年1月,广东某规模化猪场出现部分妊娠母猪流产、产死胎,为诊断发病原因,通过采集流产母猪血液、死胎脏器,应用荧光定量PCR方法检测猪圆环病毒2型/3型、猪繁殖与呼吸综合征病毒2型、猪伪狂犬病毒、弓形体等常见致母猪流产病原,并通过测序方法鉴定病原基因型及亚型;采用细菌分离鉴定方法培养细菌病原,并开展药敏试验指导临床用药。结果显示,该场流产母猪感染了猪圆环病毒2d亚型与河流漫游球菌,药敏试验显示分离的河流漫游球菌对青霉素、阿莫西林、头孢拉定、氟苯尼考均敏感。In January 2024,some pregnant sows experienced clinical cases such as miscarriage and stillbirth in a large-scale pig farm in Guangdong Province.To diagnose the cause of the outbreak,blood samples from aborting sows and organs samples from stillborn fetuses were collected.Common pathogens causing miscarriage in sows,such as Porcine Circovirus 2/3(PCV2/3),Porcine Reproductive and Respiratory Syndrome Virus 2(PRRSV2),Porcine Pseudorabies Virus(PRV),Toxoplasma gondii,etc。,have been detected using fluorescence quantitative PCR methods.Then,the genotypes and subtypes of the detected pathogens were to identify via nucleic acid sequencing analysis.In addition,the bacterial pathogens were isolated and identified,and used for screening sensitive drugs to guide clinical medication.The results showed that PCV2d and Aeromonas rivipollensis are the main pathogens causing the disease of pregnant sows in this pig farm,and the latter is sensitive to penicillin,amoxicillin,cefradine,and florfenicol,indicating that these candidate drugs can be used for clinical treatment.

关 键 词：猪圆环病毒2型 河流漫游球菌 药敏试验 

分 类 号：S852.6[农业科学—基础兽医学]