血清miRNA-214、ChE、白蛋白水平与急性有机磷中毒患者预后的关系分析  

Relationship between serum microRNA-214,ChE,albumin levels and prognosis of patients with acute organophosphate poisoning

作　　者：金善敏 魏庆 朱守仁 JIN Shanmin;WEI Qing;ZHU Shouren(Department of Emergency,Xuancheng Central Hospital,Xuancheng,Anhui 242000,China)

机构地区：[1]宣城市中心医院急诊科,安徽宣城242000

出　　处：《国际检验医学杂志》2025年第4期466-470,共5页International Journal of Laboratory Medicine

摘　　要：目的分析血清微小核糖核酸(miRNA)-214、胆碱酯酶(ChE)、白蛋白(ALB)水平与急性有机磷中毒(AOPP)患者预后的关系。方法选取2018年6月至2022年12月该院收治的160例AOPP患者作为研究对象,根据患者14 d是否存活将其分为存活组119例和死亡组41例。采用荧光定量聚合酶链反应检测两组血清miRNA-214相对表达水平,采用全自动生化分析仪检测ChE水平,免疫比浊法检测血清ALB水平。Pearson相关性分析AOPP患者血清miRNA-214与ChE、ALB的相关性。Logistic回归分析影响AOPP患者预后的危险因素,绘制受试者工作特征(ROC)曲线分析急性生理学及慢性健康状况评分系统Ⅱ(APACHEⅡ)评分、miRNA-214和ChE、ALB对AOPP患者预后的预测效能。结果死亡组AOPP患者APACHEⅡ评分、血清miRNA-214相对表达水平明显高于存活组,而ChE和ALB水平明显低于存活组,差异有统计学意义(P<0.05)。Logistic回归分析结果显示,miRNA-214≥3.75和ChE<931.92 U/L均为影响AOPP患者预后不良的独立危险因素(P<0.05)。Pearson相关性分析,死亡组AOPP患者miRNA-214和ChE、ALB无相关性(r=-0.172,0.035,P>0.05)。ROC曲线分析结果显示,APACHEⅡ评分、miRNA-214、ChE和ALB预测AOPP患者预后不良的曲线下面积分别为0.777、0.933、0.805和0.881。结论血清miRNA-214和ChE和ALB水平与AOPP患者预后有关,可作为AOPP患者预后预测的重要参考指标。Objective To analyze the relationship between serum micro ribonucleic acid(miRNA)-214,cholinesterase(ChE),albumin(ALB)levels and the prognosis of patients with acute organophosphate poisoning(AOPP).Methods A total of 160 patients with AOPP admitted to the hospital were enrolled as the research objects between June 2018 and December 2022.According to whether they were alive at 14 d after treatment,patients were divided into survival group(119 cases)and death group(41 cases).The relative expression level of serum miRNA-214 was detected by fluorescence quantitative polymerase chain reaction,ChE level was detected by full-automatic biochemical analyzer,and level of serum ALB was detected by immunoturbidimetry.The correlation between serum miRNA-214 and ChE,ALB in AOPP patients was analyzed by Pearson correlation analysis.The risk factors of prognosis in AOPP patients were analyzed by Logistic regression analysis.The predictive efficiency of acute physiology and chronic health evaluationⅡ(APACHEⅡ)score,miRNA-214,ChE and ALB for prognosis was detected by receiver operating characteristic(ROC)curves.Results APACHEⅡscore and relative expression level of serum miRNA-214 in death group were significantly higher than those in survival group,while levels of ChE and ALB were significantly lower than those in survival group(P<0.05).The results of Logistic regression analysis showed that miRNA-214≥3.75 and ChE<931.92 U/L were all independent risk factors of poor prognosis in AOPP patients(P<0.05).Pearson correlation analysis showed that miRNA-214 was not correlated with ChE and ALB in death group(r=-0.172,0.035,P>0.05).The results of ROC curves showed that area under the curve(AUC)values of APACHEⅡscore,miRNA-214,ChE and ALB for predicting poor prognosis of AOPP patients were 0.777,0.933,0.805 and 0.881,respectively.Conclusion The serum miRNA-214,ChE and ALB levels are related to prognosis of AOPP patients,which can be applied as important reference indexes to predict prognosis of AOPP patients.

关 键 词：微小核糖核酸-214 胆碱酯酶 白蛋白 急性有机磷中毒 预后 

分 类 号：R595.4[医药卫生—内科学]