鸡血藤薄层色谱鉴别与高效液相色谱指纹图谱及多指标成分含量分析  

TLC Identification,HPLC Fingerprint and Multi-components Quantitative Analysis of Spatholobus suberectus

作　　者：李立 罗轶 粟华生 莫迎 谭显骏 陈心怡 LI Li;LUO Yi;SU Huasheng;MO Ying;TAN Xianjun;CHEN Xinyi(Guangxi Zhuang Autonomous Region Institute for Drug Control,NMPA Key Laboratory for Quality Monitoring and Evaluation of Traditional Chinese Medicine,Nanning 530021,China;Guangxi Vocational University of Agriculture,Nanning 530000,China)

机构地区：[1]广西壮族自治区药品检验研究院,国家药品监督管理局中药材质量监测与评价重点实验室,南宁530021 [2]广西农业职业技术大学,南宁530000

出　　处：《医药导报》2025年第3期459-465,共7页Herald of Medicine

基　　金：广西中医药多学科交叉创新团队项目(GZKJ2309)。

摘　　要：目的建立鸡血藤薄层色谱鉴别方法、高效液相色谱(HPLC)指纹图谱以及多指标成分含量的测定方法,对不同产地鸡血藤进行质量评价。方法采用薄层色谱法对鸡血藤进行定性分析;采用HPLC指纹图谱和多种化学模式识别技术相结合方法,对鸡血藤进行质量评价;并用HPLC法对芒柄花素、表儿茶素、儿茶素、原儿茶酸、没食子儿茶素等成分含量进行分析。结果建立了鸡血藤薄层鉴别方法;在16批鸡血藤HPLC指纹图谱中鉴定了共有峰10个,并确认成分6个。指纹图谱相似度0.769~0.990,具有较好相似性。聚类分析将样品分为3类。主成分分析结果显示,综合得分排名前3位的样品分别是S16、S11、S14。正交偏最小二乘判别分析筛选了芒柄花素、峰7和表儿茶素作为不同产地鸡血藤质量差异标志物。高效液相色谱法测得没食子儿茶素、原儿茶酸、儿茶素、表儿茶素、芒柄花素等5种成分在考察的浓度范围内线性关系良好(r>0.999)。含量测定结果表明,不同产地鸡血藤中5种成分含量存在明显差异。结论所建立的鸡血藤薄层鉴别方法和HPLC指纹图谱方法具有稳定性和可靠性,多成分含量测定方法可为鸡血藤的质量监管提供科学支撑。Objective To establish TLC identification method,HPLC fingerprint,chemical pattern recognition,and multi-components quantitative analysis methods of Spatholobus suberectus,and identify the quality of 16 batches of Spatholobus suberectus from different origins.Methods The TLC method was used to identify Spatholobus suberectus qualitatively.HPLC fingerprint of Spatholobus suberectus was established.The quality of Spatholobus suberectus was evaluated by chemical pattern recognition technologies,and the contents of Gallocatechin,protocatechuic acid,catechin,epigallocatechin and fermononetin were determined by HPLC.Results TLC method of Spatholobus suberectus was established.Ten common peaks were identified in 16 batches of Spatholobus suberectus,and 6 components were identified by reference substances.The similarity of fingerprint was 0.769-0.990,indicating good similarity.The samples were divided into 3 groups by cluster analysis.Results of the principal component analysis showed that the top 3 samples in the list of comprehensive scores were S16、S11、S14.Marker compounds that cause the quality difference of Spatholobus suberectus were screened out through the orthogonal partial least squares discriminant analysis,which were fermononetin,peaks 7 and epigallocatechin.Gallocatechin,protocatechuic acid,catechin,epigallocatechin,and fermononetin had a good linearity in the concentration range(r>0.999).The content determination analysis showed that there were significant differences in the contents of the 5 index components among Spatholobus suberectus from different origins.Conclusion The established TLC and HPLC fingerprints of Spatholobus suberectus were stable and reliable,and the HPLC method for multiple active components method provides scientific support for improving the quality control method of Spatholobus suberectus.

关 键 词：鸡血藤 薄层色谱 高效液相色谱指纹图谱 化学模式识别 多指标成分 含量测定 

分 类 号：R286[医药卫生—中药学] R927.1[医药卫生—中医学]