基于网络药理学探讨三子养亲汤治疗非小细胞肺癌的作用机制  

Action mechanism of Sanziyangqin Decoction in the treatment of non-small cell lung cancer based on network pharmacology

作　　者：戴伟民 孙燕旭 汪鸣霄 李若尘 周洵 DAI Weimin;SUN Yanxu;WANG Mingxiao;LI Ruochen;ZHOU Xun(Guizhou University of Traditional Chinese Medicine,Guiyang 550005,China;ZHU Zhusheng National Famous Old Chinese Medicine Expert Inheritance Studio,Guiyang 550003,China;The Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine,Guiyang 550003,China)

机构地区：[1]贵州中医药大学,贵州贵阳550005 [2]朱祝生全国名老中医药专家传承工作室,贵州贵阳550003 [3]贵州中医药大学第二附属医院,贵州贵阳550003

出　　处：《贵州科学》2025年第1期11-16,共6页Guizhou Science

基　　金：国家中医药管理局全国名老中医药专家传承工作室建设项目(国中医药办人教函〔2022〕75号);贵州省科技厅支撑计划项目(黔科合支撑〔2021〕一般012)。

摘　　要：目的:基于网络药理学探讨三子养亲汤治疗非小细胞肺癌的作用机制。方法:运用中药系统药理数据库与分析平台(TCMSP)收集三子养亲汤的主要化学成分,根据药物口服利用度和类药性筛选药物活性成分,并获取对应靶点;从Drugbank、GeneCards和OMIM数据库获取非小细胞肺癌(NSCLC)的相关靶点,并去除重复值,使用STRING数据库进行蛋白质相互作用分析,构建PPI网络;利用Cytoscape3.7.1构建“三子养亲汤-非小细胞肺癌靶点-通路”网络。并利用Cytoscape3.7.1软件筛选出排名靠前的核心靶点;使用微生信平台对三子养亲汤与NSCLC共同靶点进行基因本体(GO)功能及京都基因和基因组(KEGG)信号通路富集分析。结果:获得106个三子养亲汤药物靶点,4083个非重复的非小细胞肺癌疾病靶点,两者取交集,共获得84个交集靶点。通过Cytoscape3.7.1获取排名前5的核心药物靶点,分别为木犀草素、β-谷甾醇、β-胡萝卜素、(+)-儿茶素、青风藤碱,通过STRING数据库分析确立TP53、ESR1、CASP3、AKT1、TNF为关键的靶点。结论:三子养亲汤通过多靶点、多途径发挥治疗非小细胞肺癌的作用。In this study the action mechanism of Sanziyangqin Decoction in the treatment of non-small cell lung cancer was explored based on network pharmacology.The main components of Sanziyangqin Decoction were collected by using the TCMSP database,the active ingredients were screened out by oral bioavailability and drug-likeness,and the related targets were obtained.NSCLC targets were obtained from the Drugbank,GeneCards and OMIM database,and the duplicate values were removed.PPI network was constructed by using the STRING database.The decoction-NSCLC targets-pathways network was constructed in Cytoscape 3.7.1 software,and the core targets were screened out.Gene ontology(GO)function and Kyoto genes and genomes(KEGG)signaling pathway enrichment were performed by online bioinformatics analysis platform.A total of 106 targets of Sanziyangqin Decoction and 4083 non-duplicated NSCLC targets were obtained,and 84 intersection targets were acquired.The top 5 core ingredients were luteolin,beta-sitosterol,beta-carotene,(+)-catechin,and sinoacutine.The core gene targets were TP53,ESR1,CASP3,AKT1,and TNF.In conclusion,Sanziyangqin Decoction exerts its therapeutic effect on NSCLC through multiple targets and pathways.

关 键 词：三子养亲汤 网络药理学 非小细胞肺癌 全国名老中医 

分 类 号：R273[医药卫生—中西医结合] G353.1[医药卫生—中医肿瘤科]