一株贵州本土黑曲霉的筛选与鉴定  

Screening and identification of an indigenous Aspergillus niger strain from Guizhou

作　　者：夏婷婷 杨仕钰 张晶 曹秀兰 邱雪松 兰立伟 燕志宏[2] XIA Tingting;YANG Shiyu;ZHANG Jing;CAO Xiulan;QIU Xuesong;LAN Liwei;YAN Zhihong(Guizhou Yuhong Biotechnology Co.Ltd.,Guiyang 550014,China;School of Animal Science,Guizhou University,Guiyang 550014,China)

机构地区：[1]贵州煜宏生物科技有限公司,贵州贵阳550014 [2]贵州大学动物科学院,贵州贵阳550014

出　　处：《贵州科学》2025年第1期41-45,共5页Guizhou Science

基　　金：基于生猪粪污生物降解地方微生物资源的研究(黔科合[2023]一般474);农业秸秆发酵床养猪技术推广应用(黔南科合[2023]24号)。

摘　　要：为筛选出一株能应用于发酵床的贵州本土黑曲霉,试验从贵州当地种植的发霉大蒜蒜瓣中筛选分离出一株菌株,通过对菌株形态特征和ITS序列进行分析鉴定,确定其分类地位,定性研究该菌株酶谱,并测定相关酶活性。结果显示,菌株与黑曲霉(Aspergillus niger)有高度同源性,确定为黑曲霉,命名为GZUAS951015,其内切葡聚糖酶酶活为538.7 U·mL^(-1)±36.4 U·mL^(-1),α-淀粉酶酶活为39.7 U·mL^(-1)±2.9 U·mL^(-1),滤纸酶酶活为29.8 U·mL^(-1)±3.8 U·mL^(-1),脂肪酶酶活为8.7 U·mL^(-1)±0.49 U·mL^(-1)。In order to screen out a strain of Aspergillus niger indigenous to Guizhou that can be applied to fermentation beds,a strain was isolated from the moldy garlic cloves grown in Guizhou,and its taxonomic status was determined by morphological characteristics and ITS sequencing.The zymogram of the strain was qualitatively studied,and the relevant enzyme activities were determined.The results showed that the strain had high degree of homology with A.niger and was identified as A.niger,named GZUAS951015.Its endoglucanase enzyme activity was 538.7 U·mL^(-1)±36.4 U·mL^(-1),α-amylase enzyme activity was 39.7 U·mL^(-1)±2.9 U·mL^(-1),filter-paper enzyme activity was 29.8 U·mL^(-1)±3.8 U·mL^(-1),and lipase enzyme activity was 8.7 U·mL^(-1)±0.49 U·mL^(-1).

关 键 词：黑曲霉 本土微生物 筛菌 酶谱 

分 类 号：S828[农业科学—畜牧学]