新工艺炮制黄精调血脂、预防动脉粥样硬化的作用研究  

Hypolipidemic and anti-atherosclerotic effects of Polygonatum processed by a new technique

作　　者：代欣 袁荣献 封帆 刘廷江 陈礼大 杨仁惠 DAI Xin;YUAN Rongxian;FENG Fan;LIU Tingjiang;CHEN Lida;YANG Renhui(The Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine,Guiyang 550003,China)

机构地区：[1]贵州中医药大学第二附属医院,贵州贵阳550003

出　　处：《贵州科学》2025年第1期61-65,共5页Guizhou Science

基　　金：贵州省科技计划项目(黔科合基础-ZK[2022]一般504)2023年度贵州省中医药;民族医药重点学科建设学科(项目编号:QZYYZDXK(JS)-2023-04)。

摘　　要：目的:探讨新工艺炮制黄精调血脂、预防动脉粥样硬化的作用。方法:取SD雄性大鼠,随机分成5组,即空白对照组、模型组、阿托伐他汀钙组(0.0009 g/kg)、新工艺炮制黄精组(1.35 g/kg)、旧黄精组(1.35 g/kg),每组10只大鼠。除空白对照组外,其余各组大鼠均灌胃高脂乳剂造模,连续4周。造模1周后,开始灌胃给药,连续3周,空白对照组、模型组灌胃等容量蒸馏水。给药结束,腹主动脉采血,检测血脂四项,并计算动脉粥样硬化指数和肝脏指数,HE染色观察肝组织病理变化。结果:与空白对照组比较,模型组大鼠的体重明显减轻,动脉粥样硬化指数、肝脏指数显著升高,血清甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL)明显增加,高密度脂蛋白胆固醇(HDL)显著降低(p<0.05,p<0.01);大鼠肝细胞脂肪变性明显。与模型组比较,各给药组大鼠体重显著增加,动脉粥样硬化指数、肝脏指数显著降低,血清TG、TC、LDL明显降低,HDL显著升高(p<0.05,p<0.01);大鼠肝细胞脂肪变性明显改善。旧黄精和新工艺炮制黄精的作用无明显的差异。结论:新工艺炮制黄精可调节脂质代谢紊乱,预防动脉粥样硬化,改善肝脏脂肪变性,且作用与旧黄精相当。In this study we explored the hypolipidemic and anti-atherosclerotic effects of Polygonatum processed by a new technique.Male SD rats were randomly allocated to five groups:blank control group,model group,atorvastatin calcium group(0.0009 g/kg),new-technique Polygonatum group(1.35 g/kg),and traditionally-processed Polygonatum group(1.35 g/kg),with 10 rats in each group.Except for the blank control group,all other groups received oral administration of high-fat emulsion for 4 continuous weeks to induce hyperlipidemia.One week after modeling,drug administration via oral gavage began for another 3 weeks.The blank control group and model group received equal volumes of distilled water.Upon completion of treatment,blood was collected from the abdominal aorta,and serum lipid profiles(four items)were analyzed.The atherosclerosis index and liver index were calculated,and HE staining was performed to assess histopathological changes in liver tissue.Compared with the blank control group,rats in the model group displayed significant weight loss,obvious rising in atherosclerosis index and liver index,significant increasing in serum levels of triglycerides(TG),total cholesterol(TC)and low-density lipoprotein cholesterol(LDL),and significant decreasing in high-density lipoprotein cholesterol(HDL)(p<0.05,p<0.01);severe hepatocellular steatosis was evident in these rats.In contrast to the model group,rats in all treatment groups showed significant weight gain,significant decreasing in atherosclerosis index,liver index,serum TG,TC and LDL levels,and significant increasing in HDL levels(p<0.05,p<0.01);hepatocellular steatosis was significantly ameliorated in these rats.There was no significant difference between the traditionally-processed Polygonatum group and the new-technique Polygonatum group.In conclusion,Polygonatum processed by the new technique can effectively regulate dyslipidemia,prevent atherosclerosis,and improve hepatic steatosis,with efficacy comparable to that of traditionally-processed Polygonatum.

关 键 词：黄精 高脂血症 动脉粥样硬化 高脂乳剂 大鼠 

分 类 号：R285[医药卫生—中药学]