基于数据挖掘技术及网络药理学探讨中药治疗输卵管性不孕用药规律及作用机制  

Discussion on Medication Rules and Mechanism of Chinese Medicinals for Tubal Infertility Based on Data Mining and Network Pharmacology

作　　者：杨程卷 段梦琦 彭思嘉 谭枚秀 YANG Chengjuan;DUAN Mengqi;PENG Sijia;TAN Meixiu(Hunan University of Chinese Medicine,Changsha Hunan 410208,China;The Second Affiliated Hospital of Hunan University of Chinese Medicine,Changsha Hunan 410005,China)

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]湖南中医药大学第二附属医院,湖南长沙410005

出　　处：《新中医》2025年第4期156-164,共9页New Chinese Medicine

基　　金：湖南中医药大学校级科研基金重点项目(2021XJJJ052)。

摘　　要：目的:基于数据挖掘技术和网络药理学探讨中药治疗输卵管性不孕(TI)的用药规律及作用机制。方法:计算机检索中国知网(CNKI)、万方数据知识服务平台(WF)、维普数据库(VIP)、PubMed等数据库,时间选择2015年1月1日—2023年9月1日,纳入符合条件的中药治疗TI的相关文献。利用Excel软件统计中药性味归经;采用R语言统计中药使用频次,并对使用频次排名前20的中药进行关联规则和聚类分析。选用聚类分析得到的核心方,借助中药系统药理学数据库与分析平台(TCMSP)获取核心方的有效成分及靶点,通过UniPort数据库进行靶点校正,在GeneCards数据库、人类孟德尔遗传数据库(OMIM)检索与TI相关的疾病基因,然后进行交集分析获得共同基因,借助Cytoscape3.10.0获得核心靶点蛋白质-蛋白质相互作用(PPI)网络图。将药物-疾病交集靶点导入Metascape数据库进行基因本体(GO)及京都基因与基因组百科全书(KEGG)富集分析。采用Cytoscape3.10.0软件构建中药-药物成分-靶点-信号通路网络图。结果:共纳入方剂120首,包含262味中药,中药总使用频次为1480次,使用频次排名前20的中药有赤芍、当归、丹参、川芎、路路通等。药性以温、寒、平性为主,药味以苦、甘、辛味居多,归经主要为肝、肾、脾经。关联规则分析得到三棱-莪术、红花-当归等药物组合。聚类分析形成6个新的药物组合,第1类药物组合(莪术、三棱、路路通、皂角刺、丹参)可能为中药治疗TI的核心方。β-谷甾醇、槲皮素、木犀草素、隐丹参酮、漆黄素等是核心方治疗TI的关键成分,这些成分可能作用于肿瘤坏死因子(TNF)、肿瘤蛋白53(TP53)、白细胞介素(IL)6、雌激素受体1(ESR1)、B淋巴细胞肿瘤2(BCL2)等靶点,并通过癌症、缺氧诱导因子-1(HIF-1)、丝裂原活化蛋白激酶(MAPK)、FoxO等信号通路发挥作用。结论:临床治疗TI多用行气活血、化瘀止痛类中�Objective:To discuss the medication rules and mechanism of Chinese medicinals for tubal infertility(TI)based on data mining and network pharmacology.Methods:The relevant literature on TI treated by Chinese medicinals were included by searching the China National Knowledge Infrastructure(CNKI),China Science Periodical Database(WF),China Science and Technology Journal Database(VIP),PubMed and other databases from January 1st,2015 to September 1st,2023.The nature,flavors and meridian entries were counted by Excel software;the use frequency of Chinese medicinals was counted by R language and the top 20 Chinese medicinals were given association rules analysis and cluster analysis.The core prescriptions based on cluster analysis were obtained and the effective ingredients and targets were worked out by using the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP).The targets were corrected by UniPort database,the TI-related disease genes were researched on GeneCards database and Online Mendelian Inheritance in Man(OMIM)database,and the common genes were figured out by intersection analysis.Using Cytoscape 3.10.0,the protein-protein interaction(PPI)networks of core targets were obtained.The medicine-disease intersection targets were imported into Metascape for enrichment analysis of Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG).The Chinese medicinals-component-target-signaling pathway networks were established by Cytoscape 3.10.0 software.Results:A total of 120 prescriptions were included,involving 262 Chinese medicinals.The Chinese medicinals were used for 1480 times in total and the top 20 included Paeoniae Radix Rubra,Angelicae Sinensis Radix,Salviae Miltiorrhizae Radix et Rhizoma,Chuanxiong Rhizoma and Liquidambaris Fructus,etc.The Chinese medicinals were mainly warm,cold and neutral in nature,and bitter,sweet and acrid in flavor,and their main meridian entries were the liver,kidney and spleen.The association rules analysis suggested the medicinal combinations

关 键 词：输卵管性不孕 数据挖掘 网络药理学 用药规律 作用机制 

分 类 号：R271.14[医药卫生—中医妇科学] R285[医药卫生—中医学]