气血并治针法对神经根型颈椎病模型大鼠海马HMGB1炎症通路的影响  

Effects of Invigorating Qi and Activating Blood EA on Hippocampal HMGB1 Inflammatory Pathway in Rats with CSR

作　　者：吴乐 嵇波[1] 刘翼天 方洋 谢亚娜 王一菲 张玲[1] 李鹤文 郭亭廷 全烨 WU Le;JI Bo;LIU Yitian;FANG Yang;XIE Yana;WANG Yifei;ZHANG Ling;LI Hewen;GUO Tingting;QUAN Ye(School of Acupuncture-Moxibustion and Tuina,Beijing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]北京中医药大学针灸推拿学院,北京100029

出　　处：《针灸临床杂志》2024年第12期79-84,共6页Journal of Clinical Acupuncture and Moxibustion

基　　金：北京中医药大学校级科研横向发展基金,编号:2030071720017。

摘　　要：目的:探讨气血并治针法对神经根型颈椎病(CSR)大鼠根性神经痛的治疗作用及中枢抗炎镇痛机制。方法:雄性SD大鼠随机分为空白对照组、假手术组、模型组和气血并治针法组,每组6只。采用椎管插线法制备CSR大鼠模型,各组于造模后3 d开始干预,电针组于“颈夹脊穴”“足三里”“膈俞”行电针刺激,强度1.5 mA,频率2/50 Hz,30 min/次,1次/d,共治疗2周。干预前1 d、干预后第3、5、7、10和14天进行行为学评价;采用HE染色观察左侧神经根病理变化;ELISA法检测海马组织IL-1β和TNF-α含量;Westen blot法检测海马组织HMGB1、TLR4及NF-κB蛋白表达情况。结果:与假手术组比较,模型组干预各时间点热痛阈值(PWTL)、机械痛阈值(PWMT)显著降低,神经根组织损伤情况较严重,受损神经根中部分神经元细胞破裂变形,细胞水肿严重,纤维排列紊乱,部分纤维断裂,胞体数量减少;海马IL-1β、TNF-α含量显著升高,差异具有统计学意义(P<0.01),HMGB1、TLR4及NF-κB蛋白表达显著上调,差异具有统计学意义(P<0.01);与模型组比较,气血并治针法组从干预第5天后PWTL显著升高,干预第7天后PWMT显著升高,神经根组织受损明显减轻,海马IL-1β、TNF-α含量显著降低,差异具有统计学意义(P<0.01),HMGB1、TLR4及NF-κB蛋白表达显著下调,差异具有统计学意义(P<0.01)。结论:气血并治针法可以缓解CSR大鼠根性神经痛,其机制可能与电针通过HMGB1信号通路抑制海马区炎症有关。Objective:To investigate the therapeutic effect of invigorating Qi and activating blood acupuncture on radicular pain and the central anti-inflammatory and analgesic mechanism in rats with cervical spondylotic radiculopathy(CSR).Methods:The male SD rats were randomly divided into the blank control group,the sham operation group,the model group and the electro-acupuncture(EA)group,with 6 rats in each group.The CSR model was established by the method of vertebral tube insertion,and the intervention started three days after modeling.The EA group was treated with electro-needling Jingjiaji(EX-B2),Zusanli(ST36)and Geshu(BL17),with the intensity of 1.5mA,frequency 2/50 Hz,30min/times,once a day for 2 weeks.Behavioral evaluations were conducted on the first day before the intervention,and the 3rd,5th,7th,10th,and 14th day after the intervention start.Pathological changes of the left nerve root were observed by HE staining.The contents of IL-1βand TNF-αin the hippocampus were detected by ELISA.The protein expressions of HMGB1,TLR4 and NF-κB in the hippocampus were detected by Western Blot.Results:Compared with those at each time point in the sham operation group,the paw withdrawal thermal latency(PWTL)and the paw withdrawal mechanical threshold(PWMT)were significantly decreased in the model group,and the nerve root tissue damage was more serious,with part of the neuronal cells ruptured and deformed,severe cell edema,disorganized fiber arrangement,part of the fibers broken,and cytosolic bodies reduced;the contents of IL-1βand TNF-αin the hippocampus were significantly increased(P<0.01),and the protein expressions of HMGB1,TLR4 and NF-κB were significantly up-regulated in the model group(P<0.01).Compared with those in the model group,the PWTL was significantly increased from the 5th day of the intervention,and the PWMT was significantly increased from the 7th day of the intervention in the EA group,with a significant reduction in the damage to the nerve root tissues,and significant decreases in the contents of hippoc

关 键 词：气血并治针法 根性神经痛 神经炎症 HMGB1通路 海马 神经根型颈椎病 

分 类 号：R246.2[医药卫生—针灸推拿学]