桃色欧文氏菌对20个紫花苜蓿品种萌发及幼苗生长的影响  

Effects of Erwinia persicina on germination and seedling growth of 20 varieties of alfalfa

作　　者：姚博 黄荣 李钰 张宇婧 张璠 张振粉[1] 师尚礼[1] YAO Bo;HUANG Rong;LI Yu;ZHANG Yu-jing;ZHANG Fan;ZHANG Zhen-fen;SHI Shang-li(College of Grassland Science,Gansu Agricultural University,Key Laboratory for Grassland Ecosystem,Ministry of Education,Grassland Engineering Laboratory of Gansu Province,Sino-U.S.Centers for Grazing Land Ecosystem Sustainability,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学草业学院,草业生态系统教育部重点实验室,甘肃省草业工程实验室,中‐美草地畜牧业可持续发展研究中心,甘肃兰州730070

出　　处：《草原与草坪》2024年第6期191-198,共8页Grassland and Turf

基　　金：国家牧草产业技术体系(CARS-34);国家自然科学基金(32060369);甘肃省杰出青年基金(20JR10RA562);甘肃省优秀研究生“创新之星”(2022CXZX-624)。

摘　　要：【目的】探究桃色欧文氏菌(Erwinia persicina)对紫花苜蓿种子的萌发和幼苗生长的影响。【方法】以20个紫花苜蓿品种为试验材料,采用培养皿内接种供试细菌的方法研究桃色欧文氏菌Cp2对紫花苜蓿种子发芽率、发芽势、发芽指数、种子活力指数以及对幼苗根长、株高、鲜重、干重以及根、茎和叶组织的影响。【结果】1)与CK组相比,菌株Cp2对20个紫花苜蓿品种的种子发芽均有抑制作用。Cp2组所有供试品种的发芽指数和种子活力指数分别显著降低13.62%~33.46%和23.10%~64.44%。2)菌株Cp2组所有紫花苜蓿品种的根长和株高较CK组分别显著降低20.68%~59.14%和4.31%~46.30%。3)菌株Cp2对20个紫花苜蓿品种幼苗的鲜重和干重均具有不同程度的降低作用。4)根据Topsis综合评价分析得出,菌株Cp2对品种巨能601、耐盐之星和威神的总体影响较小,对巨能995、旱地和皇冠3个品种的影响最大。5)菌株Cp2对品种巨能995的根、茎和叶组织细胞结构造成了损伤。【结论】本研究结果为进一步探究桃色欧文氏菌对紫花苜蓿的致病性以及紫花苜蓿抗病品种筛选提供理论依据。【Objective】The study was carried out in order to explore the effects of Erwinia persicina on the germination of alfalfa seeds and seedling growth.【Method】20 different alfalfa varieties were used as test materials.In this study,the method of inoculating the tested bacteria in a petri dish was used to study the effects of E.persicina on the germination rate,germination potential,germination index and seed vigor index of 20 varieties of alfalfa seeds,as well as the effects on seedling root length,plant length,fresh weight and dry weight.【Result】1)Compared with CK group,strain Cp2 inhibited seed germination of 20 alfalfa cultivars.The germination index and seed vigor index of all tested cultivars in Cp2 group decreased by 13.62%~33.46%and 23.10%~64.44%,respectively.2)The root length and plant height of all alfalfa cultivars in Cp2 group decreased by 20.68%~59.14%and 4.31%~46.30%,respectively.3)Strain Cp2 inhibited the fresh and dry weight of 20 alfalfa cultivars.4)According to the Topsis comprehensive evaluation and analysis,,the overall effect of E.persicina on the varieties Salt Tolerant Star,Juneng 601 and Juneng 2 was found to be low.At the same time,it had a greater effect on the varieties of Juneng 995 and Huangguan and dry land.5)Strain Cp2 damaged the cell structure of roots,stems and leaves of Juneng 995.【Conclusion】The results of this study provided a theoretical basis for further investigation of the pathogenicity of E.persicina to alfalfa and screening of resistant varieties of alfalfa.

关 键 词：桃色欧文氏菌 紫花苜蓿 种子萌发 幼苗生长 Topsis综合评价法 

分 类 号：S541.9[农业科学—作物学]