基于主成分和隶属函数分析的大豆种质资源耐盐性综合评价  

Comprehensive Evaluation of Salt Tolerance of Soybean Germplasm Resources Based on Principal Component and Membership Function Analysis

作　　者：袁宇婷 张晓燕 吴谷丰 黄璐 袁星星[2] 陈新 刘晓勇[1] 薛晨晨[1,2] YUAN Yuting;ZHANG Xiaoyan;WU Gufeng;HUANG Lu;YUAN Xingxing;CHEN Xin;LIU Xiaoyong;XUE Chenchen(School of Life Science,Jiangsu University,Zhenjiang 212013,China;Institute of Industrial Crops,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China)

机构地区：[1]江苏大学生命科学学院,江苏镇江212013 [2]江苏省农业科学院经济作物研究所,江苏南京210014

出　　处：《大豆科学》2025年第1期22-32,I0011-I0014,共15页Soybean Science

基　　金：国家重点研发计划(2023YFD23001);江苏省种业振兴揭榜挂帅项目(JBGS-2021-014);江苏现代农业产业技术体系建设专项资金(JATS[2023]025)。

摘　　要：为筛选出耐盐性较强的大豆种质资源用于盐碱地开发利用,本研究以287份大豆品种(系)为研究对象,在100 mmol·L^(-1)NaCl溶液下进行萌发期和苗期耐盐性鉴定,并利用主成分和隶属函数分析对大豆苗期的7个耐盐性相关指标(相对SPAD值、相对株高、相对根长、相对地上部鲜重、相对根鲜重、相对地上部干重和相对根干重)进行综合评价。结果表明:萌发期依据盐害指数筛选出1级耐盐种质69份,2级耐盐种质83份。苗期利用主成分分析和隶属函数对大豆材料的耐盐性进行综合评价,将287份材料分成5类:高度耐盐种质40份,耐盐种质104份,中度耐盐种质68份,盐敏感种质72份,高度盐敏感种质3份。通过逐步回归分析法建立了大豆苗期耐盐性评价的数学模型,筛选出相对地上部鲜重、相对根长、相对根干重以及相对SPAD值4个性状指标,可作为大豆苗期耐盐性的评价指标,进一步验证表明模型预测性较好(R^(2)=0.976,P<0.001)。通过对萌发期和苗期大豆耐盐性进行比较分析,筛选出萌发期与苗期均为1级耐盐的材料7份:S39(铜山白荚壳)、S149(栖霞青豆)、S154(南京高香豆)、S228(徐8107)、S262(贡豆19)、S270(ZDD 23409)以及S281(ZDD 3726)。In order to screen out soybean germplasm resources with strong salt tolerance for saline-alkali land development and utilization,287 soybean varieties(lines)were selected as the research objects,and salt tolerance at germination and seedling stage was identified under 100 mmol·L^(-1) NaCl solution.Seven indices related to salt tolerance(relative SPAD value,relative plant height,relative root length,relative shoot fresh weight,relative root fresh weight,relative shoot dry weight and relative root dry weight)were evaluated by principal component and membership function analysis.The results showed that 69 accessions with salinity tolerance of grade 1 and 83 accessions with salinity tolerance of grade 2 were selected according to salinity damage index at the germination stage.In the seedling stage,principal component analysis and membership function analysis were used to comprehensively evaluate the salt tolerance of soybean materials.The 287 accessions were divided into five categories:40 accessions with high salt tolerance,104 accessions with salt tolerance,68 accessions with moderate salt tolerance,72 accessions with salt sensitive,and three accessions with high salt sensitive.A mathematical model for evaluating salt tolerance of soybean at seedling stage was established by stepwise regression analysis.Four identification indexes,relat ive ground fresh weight,relative root length,relative root dry weight and relative SPAD value,were selected as evaluation indexes for salt tolerance of soybean at seedling stage.The prediction of the model was further verified(R^(2)=0.976,P<0.001).By comparing and analyzing the salt tolerance of soybean at germination and seedling stages,seven accessions with grade 1 salt tolerance at both seedling and germination stages were screened out,including S39(Tongshan white pod-shell),S149(Qixia green bean),S154(Nanjing Gaokou bean),S228(Xu 8107),S262(Gongdou 19),S270(ZDD 23409)and S281(ZDD 3726).

关 键 词：大豆 耐盐性 主成分分析 隶属函数 综合评价 江苏省 

分 类 号：S565.1[农业科学—作物学]