N-乙酰-L-半胱氨酸缓解盐碱胁迫对大豆幼苗生长抑制  

N-acetyl-L-cysteine Alleviates the Inhibition of Salt Alkali Stress on the Growth of Soybean Seedlings

作　　者：张欣林 李思琪 国爽 胡嘉宸 赵岩林 赵强 杜吉到[1,2] ZHANG Xinlin;LI Siqi;GUO Shuang;HU Jiachen;ZHAO Yanlin;ZHAO Qiang;DU Jidao(College of Agriculture,Heilongjiang Bayi Agricultural University,Daqing 163319,China;Heilongjiang Saline-alkali land Improvement Engineering Technology Research Center,Daqing 163319,China)

机构地区：[1]黑龙江八一农垦大学农学院,黑龙江大庆163319 [2]黑龙江省盐碱地改良工程技术研究中心,黑龙江大庆163319

出　　处：《大豆科学》2025年第1期96-105,共10页Soybean Science

基　　金：国家重点研发计划子课题“东北耐盐碱大豆耐盐碱高产生育调控关键技术”(2023YFD2300103-03);黑龙江省“双一流”新一轮建设学科协同创新成果建设项目(培育)(LJGXCG2022-111);黑龙江八一农垦大学研创项目(NXYYJSCX2022-Y05)。

摘　　要：为明确N-乙酰-L-半胱氨酸(NAC)对盐碱胁迫下大豆幼苗生长的影响,本研究以大豆品种合丰50为试验材料,采用盆栽法,通过NaCl、Na_(2)SO_(4)、NaHCO_(3)和Na_(2)CO_(3)混合溶液模拟苏打盐碱胁迫,在大豆幼苗的V3期叶面喷施1 mmol·L^(-1)NAC处理,测定盐碱胁迫下大豆幼苗的生长指标、抗氧化酶活性、活性氧含量、丙二醛含量、叶片光合指标及碳水化合物含量,以分析NAC处理对盐碱胁迫下大豆植株生长的影响。结果表明:与对照组相比,盐碱胁迫显著抑制大豆植株的生长和生物量的积累。与盐碱胁迫相比,NAC处理可显著提高大豆植株的株高、根长、茎粗、根干/鲜重和叶干/鲜重;降低叶片和根系中活性氧含量(O_(2)^(-)和H_(2)O_(2))及丙二醛(MDA)含量,叶片中MDA含量降幅达58.62%;提高叶片和根系抗氧化酶活性(POD、SOD、CAT和APX),叶片中APX增幅达82.68%;提高叶片的光合作用参数,增加叶片和根系中渗透调节物质和碳水化合物的积累。综上,NAC处理能够通过降低氧化损伤,增强大豆植株的光合能力,促进碳水化合物积累,缓解盐碱胁迫对大豆植株生长的抑制。To clarify the effect of N-Acetylcysteine on the growth of soybean seedlings under salt alkali stress,in this study,the soybean variety Hefeng 50 was used as experimental material.Pot method was used to simulate salt-alkali stress by mixing NaCl、Na_(2)SO_(4)、NaHCO_(3)andNa_(2)CO_(3),and the soybean seedlings were sprayed with 1 mmol·L^(-1) NAC on the leaf surface at the V3 stage.The growth indexes,antioxidant enzyme activity,active oxygen content,malondialdehyde content,leaf photosynthetic indexes and carbohydrate content of soybean seedlings were measured under salt and alkali stress to analyze the effects of NAC treatment on the growth of soybean plants under salt and alkali stress.The results showed that compared with the control group,salt and alkali stress significantly inhibited the growth and biomass accumulation of soybean plants.Compared with saline-alkali stress,NAC treatment could significantly increase plant height,root length,stem diameter,root dry to fresh weight and leaf dry to fresh weight.The contents of reactive oxygen species(O_(2)^(-)andH_(2)O_(2))and malondialdehyde(MDA)in leaves and roots were decreased,the content of MDA decreased by 58.62%.The activities of antioxidant enzymes(POD,SOD,CAT and APX)in leaves and roots were increased,the activities of APX enzymes imcreased by 82.68%.The photosynthetic parameters of leaves were improved,and the accumulation of osmoregulatory substances and carbohydrates in leaves and roots was increased.In conclusion,NAC treatment can enhance the photosynthetic capacity of soybean plants,promote carbohydrate accumulation,and alleviate the growth inhibition of soybean plants under saline-alkali stress by reducing oxidative damage.

关 键 词：大豆 盐碱胁迫 NAC 光合能力 碳水化合物积累 

分 类 号：S565.1[农业科学—作物学]