基于三维荧光和高通量测序解析厌氧滤池启动效能  

Analysis on Startup Performance of Anaerobic Filter Based on EEM and High-throughput Sequencing

作　　者：李娟红 黄馨玥 刘忻[2] 浩桐熙 管福征[1] LI Juan-hong;HUANG Xin-yue;LIU Xin;HAO Tong-xi;CUAN Fu-zheng(Changzhou Vocational Institute of Engineering,Changzhou,Jiangsu 213000,China;School of Environment Science&Engineering,Suzhou University of Science&Technology,Suzhou,Jiangsu 215009,China)

机构地区：[1]常州工程职业技术学院,江苏常州213000 [2]苏州科技大学环境科学与工程学院,江苏苏州215009

出　　处：《四川环境》2025年第1期138-144,共7页Sichuan Environment

基　　金：江苏省高等学校自然科学研究面上项目(19KJB610008);江苏省高职院校青年教师企业实践培训资助项目(2022QYSJPX118);常州市科技支撑计划项目(CE20212024);常州工程职业技术学院博士科研启动基金(11130900119007)。

摘　　要：为研究填料对农村污水厌氧滤池启动运行效能,以核桃壳MC和毛毡核桃壳复合填料AC为填料,研究厌氧滤池在水力停留时间18h,温度30±3℃下处理效能,利用三维荧光分析溶解性有机物DOM转化特征,并以高通量测序解析系统菌群结构。结果表明AC在19天后COD去除率为83.8%±1.5%,AC对COD和SS去除性能优于MC。三维荧光光谱显示进DOM以色氨酸蛋白和溶解性微生物代谢产物为主,出水DOM荧光峰强度降低,AC对DOM削减量高于MC。启动完成后Macellibacteroides、Clostridium sensu stricto和Syntrophomonas在AC为核心功能菌属,其相对丰度高于MC,AC和MC中主要产甲烷菌为Methanoregula,Methanolinea,Methanosphaerula和Methanospirillum,在AC中相对丰度高于MC。为快速启动厌氧滤池处理农村污水提供参考依据。In order to investigate the effects of different carriers on start-up performance of anaerobic filter during rural sewage treatment,a modified walnut shell carrier(MC)and an assembly carrier of felt and-walnut shell(AC)were used.A study was carried out in two anaerobic filters under conditions of 30±3℃ and 18h hydraulic retention time,respectively.The degradation and transformation processes of dissolved organic matter(DOM)in the two anaerobic filters was examined with EEM.In addition,the microbial community structures of two anaerobic filters were studied by means of high-throughput sequencing.The results showed that the removal rate of chemical oxygen demand(COD)was 83.8%±1.5%in AC after startup stage of 19 days.The AC showed a better removal rate of COD and SS than MC.The EEM spectra analysis revealed that the DOM in the influent was mainly associated with tryptohan protein and soluble microbial metabolites.The fluorescence peak of DOM in the effluent decreased,while AC showed a better contribution to DOM removal than MC did.In addition,the bacterial community analysis revealed that Macellibacteroides,Clostridium sensu stricto and Syntrophomonas were the core functional genus in AC,with the relative abundance greater than in those of MC at the end of the start-up stage operation.Methanoregula,Methanolinea,Methanosphaerula and Methanospirillum were the predominant methanogens in the two systems,with a greater relative abundance in AC than those in MC.The result of the research provides an important reference for quick startup of anaerobic filter to treat rural sewage.

关 键 词：厌氧滤池 填料 农村污水 三维荧光光谱 高通量测序 

分 类 号：X703[环境科学与工程—环境工程]