金银花中多组分同步检测与质量综合评价方法的研究  

Study on Simultaneous Detection and Comprehensive Quality Evaluation of Multi-Component in Jinyinhua(Lonicera Japonica Flos)

作　　者：赵云平[1] 赵琳琳[1] 魏静娜[1] 焦艺博[2] 苏蘩 孙萌 刘征辉 ZHAO Yunping;ZHAO Linlin;WEI Jingna;JIAO Yibo;SU Fan;SUN Meng;LIU Zhenghui(Tianjin High-standard Quality Testing Lab.,Tianjin 300081,China;General Hospital of Tianjin Medical University,Tianjin 300052,China;Tianjin Academy of Agricultural Sciences,Tianjin 300081,China)

机构地区：[1]天津海世达检测技术有限公司,天津300381 [2]天津医科大学总医院,天津300052 [3]天津市农业科学院,天津300381

出　　处：《辽宁中医杂志》2025年第2期132-135,I0001,共5页Liaoning Journal of Traditional Chinese Medicine

基　　金：天津市农业农村委员会产学研用合作“揭榜挂帅”项目(GBGG202302);天津市科技成果转移转化优秀农业科技特派员项目(23ZYCGSN00470)。

摘　　要：目的研究建立金银花中多组分的同步检测与质量综合评价的方法。方法通过超高效液相色谱法,采用Waters ACQUITY BEH C 18(100 mm×2.1 mm,1.7μm)色谱柱,流动相为乙腈-0.1%磷酸水溶液,洗脱方式为梯度洗脱,流速为0.2 mL/min,柱温35℃。结果建立了超高效液相色谱法同时测定金银花中8种组分含量的方法,并结合指纹图谱和聚类分析对金银花的质量进行综合评价。结论建立的超高效液相色谱分析方法简便快速,节省了检测时间,提高了检测效率;指纹图谱分析结果证明了蓟州和津南种植的金银花所含组分比较一致,相似度高,组分含量通过聚类分析加以区分。Objective To establish a method for simultaneous detection and comprehensive quality evaluation of multiple components in Jinyinhua(Lonicera Japonica Flos).Methods Ultra-high performance liquid chromatography was performed on Waters ACQUITY BEH C 18(100 mm×2.1 mm,1.7μm)column with mobile phase consisting of acetonitrile-0.1%phosphoric acid aqueous solution.Gradient elution was performed at the flow rate of 0.2 mL/min.The column temperature was 35℃.Results A method for simultaneous determination of 8 components in Jinyinhua(Lonicera Japonica Flos)by ultra-high performance liquid chromatography was established,and the quality of Jinyinhua(Lonicera Japonica Flos)was evaluated comprehensively by combining fingerprint and cluster analysis.Conclusion The established ultra-high performance liquid chromatography method is simple and rapid,saving the detection time and improving the detection efficiency.The results of fingerprint analysis proved that the components of honeylonicles planted in Jizhou and Jinnan were consistent and highly similar,and the component contents were distinguished by cluster analysis.

关 键 词：金银花 多组分 指纹图谱 聚类分析 质量评价 

分 类 号：R284[医药卫生—中药学]