基于LTB4/BLT1/NF-κB信号通路研究阿魏酸对银屑病的抗炎机制  

Study on Anti-Inflammatory Mechanism of Ferulic Acid Against Psoriasis Based on LTB4/BLT1/NF-κB Signaling Pathway

作　　者：徐瑶 孙雪淞 薛宇 张丽宏[1] XU Yao;SUN Xuesong;XUE Yu;ZHANG Lihong(Heilongjiang University of Chinese Medicine,Harbin 150000,Heilongjiang,China)

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150000

出　　处：《辽宁中医杂志》2025年第2期177-180,共4页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81973846);黑龙江省卫生计生委科研项目(2017-577);黑龙江中医药大学研究生创新科研项目立项(2023yjscx016)。

摘　　要：目的探讨阿魏酸对LPS诱导的巨噬细胞RAW264.7银屑病炎症模型的保护作用及其机制,明确LTB4/BLT1/NF-κB信号通路在此过程中的作用。方法将RAW264.7巨噬细胞分组培养,分为空白组(Control组)、模型组(Model组)、阿魏酸组(FA组)、齐留通组(Zileuton组)、齐留通+阿魏酸组(Zileuton+FA组)。在处理每组24 h后收集细胞和细胞培养液。采用酶联免疫法测量LTB4表达量,采用蛋白质免疫印迹法测定5-LOX、BLT1、P-65、p-P65蛋白表达量。并用5-LOX的抑制剂齐留通来研究阿魏酸抗银屑病炎症的作用机制。结果与Model组相比,FA组5-LOX(P<0.01)、LTB4(P<0.01)、BLT1(P<0.01)含量,p-P65/P65比值(P<0.05)均显著降低。Zileuton组5-LOX含量高于Control组(P<0.01),低于Model组(P<0.01);LTB4含量高于Control组(P<0.05),低于Model组(P<0.01);BLT1表达量低于Model组(P<0.01),且高于Control组,差异无统计学意义;p-P65/P65比值高于Control组,差异无统计学意义,低于Model组(P<0.05)。Zileuton+FA组5-LOX含量高于Control组(P<0.01),低于Model组,且差异无统计学意义;LTB4含量高于Control组(P<0.05),低于Model组(P<0.01);BLT1表达量均低于Model组(P<0.01),且高于Control组,差异无统计学意义;p-P65/P65比值高于Control组,差异无统计学意义,低于Model组(P<0.05)。结论阿魏酸对LTB4/BLT1/NF-κB信号活性的抑制作用可能是其防治银屑病机制之一。Objective To explore the protective effect and mechanism of ferulic acid on lipopolysaccharide(LPS)-induced inflammatory model of macrophage RAW264.7 psoriasis,and to clarify the role of leukotriene B4(LTB4)/leukotriene B4 receptor 1(BLT1)/nuclear factor kappa-B(NF-κB)signaling pathway in this process.Methods The RAW264.7 macrophages were cultured and divided into blank group(Control group),Model group,ferulic acid(FA)group,Zileuton group and Zileuton+FA group.Cells and cell culture solution were collected after 24 hours of treatment in each group.The expression of LTB4 was measured by enzyme-linked immunosorbent assay,and the expressions of 5-lipoxygenase(5-LOX),BLT1,P-65 and p-P65 proteins were measured by protein Western blot.Zileuton,an inhibitor of 5-LOX,was used to study the anti-inflammatory mechanism of FA.Results Compared with those of the Model group,the contents of 5-LOX(P<0.01),LTB4(P<0.01)and BLT1(P<0.01)and the ratio of p-P65/P65(P<0.05)in FA group were significantly decreased.The content of 5-LOX in the Zileuton group was higher than that in the Control group(P<0.01)and lower than that in the Model group(P<0.01).The content of LTB4 was higher than that of the Control group(P<0.05)and lower than that of the Model group(P<0.01).The expression of BLT1 was lower than that of the Model group(P<0.01)and higher than that of the Control group,with no statistical significance.The ratio of p-P65/P65 was higher than that in the Control group,but lower than that in the Model group(P<0.05).The content of 5-LOX in the Zileuton+FA group was higher than that in the Control group(P<0.01)and lower than that in the Model group,and there was no statistical significance.The content of LTB4 was higher than that of the Control group(P<0.05)and lower than that of the Model group(P<0.01).The expression of BLT1 was lower than that of the Model group(P<0.01)and higher than that of the Control group,with no statistical significance.The ratio of p-P65/P65 was higher than that in the Control group,but lower than that in the Mo

关 键 词：阿魏酸 银屑病 白三烯 5-LOX LTB4 BLT1 NF-κB 

分 类 号：R285.5[医药卫生—中药学]