Pattern-based rapid and robust profiling of gut microbiota and its metabolism with a single fluorescent probe  

作　　者：Hongli Tan Meizi Zeng Cong Fang Xiaohua Zhu Feng Liu Ying Long Ruijie Deng Peng Yin Meiling Liu Haitao Li Youyu Zhang Shouzhuo Yao 

机构地区：[1]Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research(Ministry of Education),College of Chemistry and Chemical Engineering,Institute of Interdisciplinary Studies,Hunan Normal University,Changsha 410081,China [2]Hunan Cancer Hospital/The Affiliated Cancer Hospital of Xiangya School of Medicine,Central South University,Changsha 410013,China [3]College of Biomass Science and Engineering,Healthy Food Evaluation Research Center,Sichuan University,Chengdu 610064,China

出　　处：《Science China Chemistry》2025年第2期781-788,共8页中国科学(化学英文版)

基　　金：supported by the National Natural Science Foundation of China(22376057,22274048,22274045,22074100,22274047,22104033);the Foundation of the Science&Technology Department of Hunan Province(2021ZJ1123,2023JJ30394);support from China Postdoctoral Science Foundation(2023M731053)。

摘　　要：The gut microbiota and the associated metabolism play a pivotal role in maintaining human health,yet their assessment is challenging due to their inherent diversity,variety,and complexity.Herein,we developed a multichannel sensor array based on a single fluorescent probe,allowing for rapid and robust profiling gut microbiota and their key metabolites including cysteine(Cys),glutathione(GSH),and homocysteine(Hcy).The assay leverages the single fluorescent probe that is with multiple binding sites and the cross-reactive sensing principle to specifically recognize different biological thiols.By analyzing the pattern of biological thiols,the assay is capable of rapidly identifying six gut-derived bacteria including probiotics,neutral,and pathogenic strains based on fluorescent fingerprints within 5 min,and also discriminating bacteria and their mixtures with different composition.Using the assay that enables the simultaneous measurement of multiple gut-derived bacteria and their metabolites,the designed array achieved an accuracy of 0.99 when discriminating colorectal cancer(CRC)patient feces samples from healthy individuals.Remarkably,the as-prepared sensor array can also be used to identify various stages of CRC.The simplicity,rapidness,and cost-effectiveness of our approach render it a robust platform for the analysis of gut microbiota.

关 键 词：gut microbiota biological thiols sensor array fluorescent probe 

分 类 号：R446[医药卫生—诊断学] O657.3[医药卫生—临床医学]