血液透析对外周组织中氧代二十碳四烯酸生物转化的影响  

Effect of hemodialysis on the biotransformation of oxo-eicosatetraenoic acids in peripheral tissues

作　　者：刘彤 Maik Gollasch Friedrich C.Luft 林攀 吉俊 孟尧 LIU Tong;Maik Gollasch;Friedrich C.Luft;LIN Pan;JI Jun;MENG Yao(Department of Nephrology,Zhongshan Hospital,Fudan University,Shanghai 200032,China;Department of Nephrology,Shanghai Geriatrics Medical Center,Shanghai 201100,China;Department of Internal Medicine and Geriatrics,University Medicine Greifswald,Greifswald 17475,Germany;Experimental and Clinical Research Center,a joint institution of CharitéMedical Faculty and Max Delbrück Center(MDC)for Molecular Medicine,Berlin 13125,Germany)

机构地区：[1]复旦大学附属中山医院肾内科,上海200032 [2]上海市老年医学中心肾内科,上海201100 [3]格赖夫斯瓦尔德大学医学院内科和老年病学系,格赖夫斯瓦尔德174754 [4]夏里特医学院与马克斯-德尔布吕克分子医学中心联合机构,实验与临床研究中心,柏林13125

出　　处：《中国临床医学》2025年第1期93-100,共8页Chinese Journal of Clinical Medicine

摘　　要：目的分析血液透析(hemodialysis,HD)患者血细胞和血浆中不同酯化状态氧代二十碳四烯酸(oxoeicosatetraenoic acids,oxo-ETEs)的动静脉含量差异。方法收集2020年6月至12月德国柏林夏里特医院12例终末期肾病(end-stage renal disease,ESRD)患者接受HD治疗前后的外周动脉血和静脉血样本。采用高效液相色谱-串联质谱(high performance liquid chromatography-tandem mass spectrometry,HPLC-MS/MS)法测量血细胞和血浆中花生四烯酸衍生的酯化和游离oxo-ETEs的丰度。结果HD治疗前后,血细胞中酯化和游离oxo-ETEs均未显示明显的动静脉含量差异。HD主要影响血浆中酯化和游离oxo-ETEs的代谢水平。HD缩小了血浆中酯化12-oxo-ETE、游离15-oxo-ETE和游离5-oxo-ETE的动静脉含量差异,增加了酯化15-oxo-ETE的动静脉含量差异。结论血细胞中oxo-ETEs对HD的反应相对稳定,血浆中游离和酯化oxo-ETEs受到HD影响,这可能对HD患者心血管事件的发生产生不利影响。Objective To analyze the differences of free and esterified oxo-eicosatetraenoic acids(oxo-ETEs)in blood cells and plasma from arterial and venous blood in hemodialysis(HD)patients.Methods Arterial and venous blood samples from 12 patients with end-stage renal disease(ESRD)before and after HD treatment at Charité–Universitätsmedizin Berlin,Germany,from June to December 2020 were collected.The esterified and free oxo-ETEs derived from arachidonic acid in blood cells and plasma were measured by high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS).Results Neither esterified nor free oxo-ETEs in blood cells displayed significant arteriovenous differences before and after HD.HD predominantly affected the metabolic levels of esterified and free oxo-ETEs in plasma.HD reduced the arteriovenous differences of esterified 12-oxo-ETE,free 15-oxo-ETE,and free 5-oxo-ETE in plasma,while raised the arteriovenous differences of esterified 15-oxo-ETE.Conclusions The oxo-ETEs in blood cells are relatively well-stabilized responding to HD treatment,whereas arteriovenous differences of free and esterified oxo-ETEs in plasma are present and active in response to HD treatment,potentially contributing to the cardiovascular disease.

关 键 词：血液透析 类二十烷酸 氧代二十碳四烯酸 生物转化 动静脉差异 

分 类 号：R692.5[医药卫生—泌尿科学]