温阳补肾灸对APP/PS1双转基因小鼠突触可塑性的影响  

Effect of Wenyang Bushen Moxibustion on Synaptic Plasticity in APP/PS1 Double Transgenic Mice

作　　者：崔倩倩[1] 朱才丰[1] 郑仕平[1] 葛侠[1] 杨成[1] 贺成功[1] 贾玉梅 CUI Qianqian;ZHU Caifeng;ZHENG Shiping;GE Xia;YANG Cheng;HE Chenggong;JIA Yumei(The Second Affiliated Hospital of Anhui University of Chinese Medicine,Hefei 230061,China;Anhui University of Chinese Medicine,Hefei 230012,China)

机构地区：[1]安徽中医药大学第二附属医院,安徽合肥230061 [2]安徽中医药大学,安徽合肥230012

出　　处：《针灸临床杂志》2025年第2期83-88,共6页Journal of Clinical Acupuncture and Moxibustion

基　　金：安徽省自然科学基金,编号:2208085MH273;安徽中医药大学校自然科学研究项目,编号:2020efyzc10;安徽省卫生健康委科研项目,编号:AHWJ2021b033;周氏梅花针灸流派工作室,编号:皖中医药发展秘(2021)30号;蔡圣朝名医工作室,编号:国中医药人教发[2014]20号;安徽省高等学校科学研究项目,编号:2022AH050516。

摘　　要：目的:观察温阳补肾灸对APP/PS1双转基因小鼠突触可塑性损伤及Aβ的影响,探讨温阳补肾灸治疗阿尔茨海默病(AD)的作用机制。方法:10只C57BL/6J小鼠作为正常组;20只APP/PS1双转基因小鼠随机分为模型组和艾灸组,各10只。艾灸组悬灸“百会穴”“命门穴”,隔龟板灸“关元穴”,每次30 min,1次/d,共3周。Morris水迷宫实验检测干预前后小鼠学习记忆能力;Western blot检测线粒体分裂蛋白1(Fis1)、线粒体融合蛋白1(OPA1)、突触后致密物-95(PSD-95)和突触素(SYN)的蛋白表达;透射电镜观察突触形态结构;免疫组织化学法检测β-淀粉样蛋白_(1-42)(Aβ_(1-42))蛋白表达。结果:干预前,模型组与正常组比较逃避潜伏期时间延长,差异具有统计学意义(P<0.05);模型组与正常组比较,Fis1和Aβ_(1-42)蛋白表达均升高,差异具有统计学意义(P<0.05),OPA1、PSD-95和SYN蛋白表达均下降,差异具有统计学意义(P<0.05)。电镜下观察模型组的突触超微结构损伤,突触间隙增宽。干预后,艾灸组与模型组比较逃避潜伏期时间缩短,差异具有统计学意义(P<0.05);Fis1和Aβ_(1-42)蛋白均表达下降,差异具有统计学意义(P<0.05);OPA1、PSD-95和SYN蛋白表达均升高,差异具有统计学意义(P<0.05);电镜下观察艾灸组突触形态结构改善,突触间隙宽度减小。结论:温阳补肾灸通过抑制Aβ沉积减少线粒体损伤,改善突触可塑性损伤,进而改善认知功能。Objective:To observe the effect of Wenyang Bushen(warming Yang and tonifying Kidney)moxibustion on synaptic plasticity damage and Aβin APP/PS1 double transgenic mice,thus to explore the mechanism of Wenyang Bushen moxibustion in treatment of Alzheimer's disease(AD).Methods:10 C57BL/6J mice were used as the normal control.20 APP/PS1 mice were randomly divided into the model group and the moxibustion group,with 10 mice in each group.The moxibustion group used suspension moxibustion on Baihui(DU20)and Mingmen(DU04),and turtle plate moxibustion on Guanyuan(RN4)for 30 minutes,once per day for three weeks.The learning-memory ability was detected by Morris water maze tests in the mice.The protein expressions of Fis1,OPA1,PSD-95 and SYN were detected by Western blot.Morphological structure of synapses was observed by transmission electron microscopy.The protein expression of Aβ_(1-42)was detected by immunohistochemistry method.Results:The escape latency time of the model group was prolonged compared to the normal group(P<0.05).Compared with those in the normal group,the protein expressions of Fis1 and Aβ_(1-42)were increased(P<0.05),and the protein expressions of OPA1,PSD-95 and SYN were decreased in the model group(P<0.05).Under electron microscopy,the model group showed synaptic ultrastructural damage,including widened synaptic clefts.After the intervention,the moxibustion group demonstrated a shortened escape latency compared to the model group(P<0.05).The protein expressions of Fis1 and Aβ_(1-42)were decreased(P<0.05),and the protein expressions of OPA1,PSD-95,and SYN were increased in the moxibustion group(P<0.05).Under electron microscopy,the moxibustion group exhibited improved synaptic morphology,with a reduced synaptic cleft width.Conclusion:Wenyang Bushen moxibustion reduces mitochondrial damage by inhibiting Aβ_(1-42)deposition,which in turn improves synaptic plasticity impairment and enhances cognitive function.

关 键 词：阿尔茨海默病 温阳补肾灸 学习认知能力 线粒体损伤 突触可塑性 海马 

分 类 号：R246.6[医药卫生—针灸推拿学]