利用核心SNP标记构建豆类蔬菜品种指纹图谱  

Construction of DNA fingerprinting of vegetable legumes using core-SNP markers

作　　者：吴新义[1,2] 刘娜 汪宝根[2] 龚亚明[2] 李国景[2] WU Xinyi;LIU Na;WANG Baogen;GONG Yaming;LI Guojing(State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,Zhejiang;Institute of Vegetables,Zhejiang Academy of Agricultural Sciences,Key Laboratory of Vegetable LegumesGermplasm Enhancement and Molecular Breeding in Southern China(Co-construction by Ministry and Province),Ministry of Agriculture and Rural Affairs,Hangzhou 310021,Zhejiang)

机构地区：[1]浙江省农业科学院省部共建农产品质量安全危害因子与风险防控国家重点实验室,浙江杭州310021 [2]浙江省农业科学院蔬菜研究所农业农村部南方豆类蔬菜种质创新与分子育种重点实验室(部省共建),浙江杭州310021

出　　处：《浙江农业科学》2025年第2期363-369,共7页Journal of Zhejiang Agricultural Sciences

基　　金：浙江省农业新品种选育重大专项(2021C02065-6-3);浙江省重点研发计划(2022C02016)。

摘　　要：豆类蔬菜是我国重要的蔬菜作物。为促进豆类蔬菜品种权保护,以豇豆和菜用豌豆为研究对象,分别利用物种特异的核心单核苷酸多态性(SNP)标记构建了52份豇豆品种(系)和48份豌豆种质的DNA指纹图谱,并对14份豇豆品种(系)的种子纯度进行了检测。该研究结果表明,基于竞争性等位基因特异性PCR(KASP)技术的SNP标记可以用于豆类蔬菜品种的品种权保护、种子真伪区分以及种子纯度鉴定,建议尽快制订并发布基于SNP标记的豆类蔬菜品种鉴定技术标准,为种业和产业健康发展提供技术支撑。Vegetable legumes are important vegetable crops in China.To facilitate the variety protection of vegetable legumes,this study focused on cowpea and vegetable pea as research objects,and constructed the DNA fingerprinting maps of 52 cowpea varieties(lines)and 48 pea germplasm using the species-specific core single nucleotide polymorphism(SNP)markers,respectively.We also evaluated the seed purity for 14 cowpea varieties(lines).Our results showed that SNP markers based on the kompetitive allele-specific PCR(KASP)technology could be used in protecting the new variety rights,distinguishing the variety authenticity and evaluating the seed purity.Meanwhile,we also suggested that we should formulate and publish the technical standards for identifying the vegetable legumes varieties based on SNP markers as soon as possible,which will provide the technical support for the healthy development of seed industry in vegetable legumes.

关 键 词：豆类蔬菜 SNP标记 KASP 品种保护 种子纯度 

分 类 号：S643[农业科学—蔬菜学]