基于16S rDNA扩增子分析柑橘黄龙病植株叶片中脉内生细菌菌群  

Analysis of endophytic bacterial populations in the midvein of citrus leaves infected with Huanglongbing based on 16S rDNA amplicons

作　　者：张利平[1] 刘顺民 蒲占湑[1] 朱莉 吕佳 ZHANG Liping;LIU Shunmin;PU Zhanxu;ZHU Li;LYU Jia(Zhejiang Citrus Research Institute,Taizhou 308026,Zhejiang)

机构地区：[1]浙江省柑橘研究所,浙江台州308026

出　　处：《浙江农业科学》2025年第2期440-444,共5页Journal of Zhejiang Agricultural Sciences

基　　金：台州市项目(15ny14)。

摘　　要：文章以黄龙病菌亚洲种为样本分析柑橘黄龙病植株叶片中脉的内生细菌种群,通过对发病样本(HLB)总共24个,空白样本(HC)12个,进行16S rDNA V3-V4区扩增子测序,通过生物信息学分析得到60种有注释的微生物OTU(可认为是60种微生物),发现HC组有54种OTU,HLB组只有38种OTU,其中HC组注释到属水平的有38个属,HLB组有30个属,在HLB组中,黄龙病菌(Candidatus Liberibacter)是优势菌群,平均占比达到81.35%,而在HC组中并未发现黄龙病菌。通过t-test分析发现,发病组和健康组中的Cadidatus Liberibacter和Roseburia2个微生物的占比在属水平上具有显著差异;根据Spearman秩相关性分析发现黄龙病菌与拟杆菌属(Bacteroides)、梭形杆菌属(Fusobacterium)及罗氏菌属(Roseburia)3个属呈正相关,与鞘氨醇单胞菌属(Sphingomonas)、放线菌属(Amnibacterium)、甲基杆菌属(Methylobacterium)及Beijerinckiaceae 1174-901-12这4个属呈负相关。In this paper,the endophytic bacterial populations were analyzed in the midvein of the citrus leaves infected with Candidatus Liberibacter asiaticus which was the agent of citrus Huanglongbing.By conducting 16S rDNA V3-V4 region amplicon sequencing on a total of 24 infected samples(HLB)and 12 control samples(HC)and bioinformatics analysis,and filtering identified 60 annotated microbial OTUs(considered as 60 different microorganisms)were identified.It was found that the HC group had 54 OTUs,while the HLB group only had 38 OTUs.Among these,the HC group had 38 genera annotated at the genus level,while the HLB group had 30 genera.Candidatus Liberibacter was the dominant microrganism with an average percentage of 81.35%in the HLB group,Candidatus Liberibacter was not found in the HC group.Percentages of Candidatus Liberibacter and Roseburia in total were found to differ significantly between HC and HLB groups by t-test analysis.According to Spearman's rank correlation analysis,Candidatus Liberibacter was found to be positively correlated with three genera,Bacteroides,Fusobacterium and Roseburia,and negatively correlated with four genera,Sphingomonas,Amnibacterium,Methylobacterium and Beijerinckiaceae 1174-901-12.

关 键 词：柑橘黄龙病 16S rDNA 内生细菌 

分 类 号：S436.66[农业科学—农业昆虫与害虫防治]