Polycaprolactone strengthening gelatin/nano-hydroxyapatite composite biomaterial inks for potential application in extrusion-based 3D printing bone scaffolds  

作　　者：Chenxin Wang Mao Yang Li Chen Yijing Stehle Mingyue Lin Rui Zhang Huanshuo Zhang Jiehui Yang Min Huang Yubao Li Qin Zou 

机构地区：[1]Analytical&Testing Center,Sichuan University,Chengdu 610064,P.R.China [2]Sichuan University Wangjiang Hospital,Sichuan University,Chengdu 610064,P.R.China [3]Department of Mechanical Engineering,Union College,807 Union St,Schenectady,NY 12308,USA [4]Sichuan Institute of Atomic Energy,Chengdu 610101,P.R.China

出　　处：《Collagen and Leather》2024年第3期119-140,共22页胶原与皮革(英文)

基　　金：supported by the National Key Research and Development Program of China[No.2021YFA1201300];Innovation and Reform Project of Postgraduate Education of Sichuan University in 2021;Analysis and Test Technology Innovation Project of Analytical&Testing Center

摘　　要：Extrusion-based three-dimensional(3D) printing of gelatin(Gel) is crucial for fabricating bone tissue engineering scaffolds via additive manufacturing. However, the thermal instability of Gel remains a persistent challenge, as it tends to collapse at mild temperatures. Current approaches often involve simply mixing Gel particles with various materials, resulting in biomaterial inks that lack uniformity and have inconsistent degradation characteristics. In this study, acetic acid was used to dissolve Gel and polycaprolactone(PCL) separately, producing homogeneous Gel/PCL dispersions with optimal pre-treatment performance. These dispersions were then combined and hybridized with nano-hydroxyapatite(n-HA) to create a composite printing ink. By evaluating the printability of the ink, the optimal conditions were identified: a n-HA concentration of 50%(w/w), a printing temperature of 10–15 ℃, a printing pressure of 2.5 bar, and a printing speed of 7 mm/s. The resulting biomaterial inks, with a composition of 25% Gel, 25% PCL, and 50% n-HA, demonstrated excellent printability and stability, along with significantly enhanced mechanical properties. As a result, 3D scaffolds with high printability and shape fidelity can be printed at room temperature, followed by deep freezing at-80 ℃ and cross-linking with vanillin. The Gel-based composite scaffolds demonstrated excellent biocompatibility, cell adhesion, cell viability and nano-hydroxyapatite absorption in vitro. Additionally, in vivo experiments revealed that the bioactive scaffold biodegraded during implantation and significantly promoted bone regeneration at the defect site. This provides a promising strategy for treating bone defects in clinical setting. In conclusion, the Gel/PCL/n-HA biomaterial inks presented here offer an innovative solution for extrusion bioprinting in the field of bone tissue engineering.

关 键 词：GELATIN NANO-HYDROXYAPATITE POLYCAPROLACTONE Polymer-matrix composites scaffold(PMCs) Bone regeneration 

分 类 号：R31[医药卫生—基础医学]