维生素C和甘草酸联合防治新型冠状病毒感染的作用机制研究  

Study on the mechanism of vitamin C and glycyrrhizin acid in co-treatment of COVID-19

作　　者：郭永梅 徐蕊 GUO Yong-mei;XU Rui(College of Pharmacy,Jiangxi Medical College,Shangrao,334000,China)

机构地区：[1]江西医学高等专科学校药学院,江西上饶334000

出　　处：《云南民族大学学报(自然科学版)》2025年第1期68-76,共9页Journal of Yunnan Minzu University:Natural Sciences Edition

基　　金：江西省卫健委科技项目(SKJP220225895)。

摘　　要：基于网络药理学技术,探索维生素C(VC)和甘草酸(GA)联合防治新型冠状病毒感染(COVID-19)的作用机制.通过中药系统药理学数据库与分析平台(TCMSP)、SuperPred、SEA Search Server(SEA)、DrugBank、Comparative Toxicogenomics Database(CTD)和SwissTargetPrediction数据库综合相关文献获得VC和GA功能靶标,以及Genecards等数据库获取COVID-19治疗靶点;构建“药物-疾病-靶点”网络,并进行基因本体论(GO)富集分析、京都基因与基因组百科全书(KEGG)通路分析和蛋白互作网络分析(PPI);运用Autodock Vina软件进行分子对接.筛选出VC功能靶标667个、GA功能靶标154个、COVID-19靶点1 586个,VC与GA共有45个靶标与COVID-19致病靶点相关.关键靶点包括ALB、MAPK3、EGFR、IL2、MAPK1和CTLA4等. GO分析显示靶点富集于肽基丝氨酸磷酸化、蛋白结合等生物过程,KEGG分析提示靶点主要作用于5-羟色胺能突触通路.分子对接表明,VC和GA与SARS-CoV-2 3CL水解酶、ACE2等靶点具有较强结合能力.研究表明,VC和GA可能通过调节免疫系统的激活,在COVID-19预防和治疗中发挥协同作用.Utilizing network pharmacology technology,this study investigated the mechanism of vitamin C(VC)and glycyrrhizic acid(GA)in prevention and treatment of COVID-19.The functional targets of VC and GA were obtained through databases from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP),SuperPred,SEA Search Server(SEA),DrugBank,Comparative Toxicogenomics Database(CTD),and SwissTargetPrediction.The therapeutic targets for COVID-19 were obtained from databases such as GeneCards.A“drug-disease-target”network was constructed,followed by Gene Ontology(GO)enrichment analysis,Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis,and protein-protein interaction(PPI)network analysis.Molecular docking simulations were performed using Autodock Vina software.A total of 667 functional targets for VC,154 functional targets for GA,and 1,586 therapeutic targets for COVID-19 were identified.There were 45 overlapping targets between VC and GA that are relevant to the pathogenic mechanisms of COVID-19.Key targets included ALB,MAPK3,EGFR,IL2,MAPK1,and CTLA4.GO analysis revealed that these targets were enriched in biological processes such as peptidyl serine phosphorylation and protein binding.KEGG analysis indicated that the targets primarily acted on pathways like the 5-hydroxytryptamine(serotonin)syn-aptic pathway.Molecular docking results demonstrated that both VC and GA exhibited strong binding affinities with critical targets such as SARS-CoV-23CL protease and ACE2.This study suggests that VC and GA may exert synergistic effects in the prevention and treatment of COVID-19 by modulating immune system activation.

关 键 词：新型冠状病毒感染 维生素C 甘草酸 网络药理学 作用机制 

分 类 号：R285[医药卫生—中药学]