鼠伤寒沙门菌greA/B基因缺失株的构建及生物学特性  

Construction and biological characterization of greA/B-deleted Salmonella Typhimurium strains

作　　者：叶景芬 陈世雄 武绍碧 杨婉 潘永 廖怡雯 罗雪 杨琦[1,2] YE Jingfen;CHEN Shixiong;WU Shaobi;YANG Wan;PAN Yong;LIAO Yiwen;LUO Xue;YANG Qi(School of Animal Science,Guizhou University,Guiyang,Guizhou,China;Institute of Animal Diseases,Guizhou University,Guiyang,Guizhou,China;Institute of Animal Husbandry and Veterinary Medicine,Guizhou Academy of Agricultural Sciences,Guiyang,Guizhou,China)

机构地区：[1]贵州大学动物科学学院,贵州贵阳 [2]贵州大学,动物疫病研究所,贵州贵阳 [3]贵州省农业科学院畜牧兽医研究所,贵州贵阳

出　　处：《微生物学报》2025年第2期644-654,共11页Acta Microbiologica Sinica

基　　金：国家自然科学基金(32260884);贵州省科技支撑计划(黔科合支撑[2024]一般086号)。

摘　　要：沙门菌作为一种常见的人畜共患病原体,可导致多种食源性疾病,其中鼠伤寒沙门菌(SalmonellaTyphimurium,STM)是关键血清型之一,其研究与防控对公共卫生具有重要意义。【目的】探究greA和greB基因对鼠伤寒沙门菌生物学特性和致病性的影响。【方法】运用Red同源重组技术构建greA和greB基因缺失株及其回补株,并对其生长特性、生物被膜形成能力、对Caco-2细胞的黏附及侵袭能力进行检测;利用小鼠模型评估了greA和greB基因缺失对STM致病性的影响。【结果】成功构建了突变株STM LT2ΔgreA和STM LT2ΔgreB;与野生株相比,greA和gre B基因的缺失均不影响其生长速度,但导致STM的生物被膜形成能力、黏附力、侵袭力均有所下降;greA和greB缺失降低了STM对小鼠肝、脾的定殖力,并使得STM的LD50分别上升了39.81倍和2.5倍。【结论】greA和greB基因的缺失均可降低鼠伤寒沙门菌的致病性,本研究结果为进一步揭示沙门菌的致病机理提供了理论基础。Salmonella as common zoonotic pathogens can cause a variety of foodborne diseases.Salmonella Typhimurium(STM)is one of the key serotypes,and the research,prevention,and control of STM are of great significance to public health.[Objective]To investigate the effects of greA and greB on the biological characteristics and pathogenicity of STM.[Methods]Red homologous recombination was employed to construct the greA-and greB-deleted STM strains well as the complemented strains.The strains were then characterized in terms of growth characteristics,biofilm formation,and adhesion and invasion in Caco-2 cells.Mouse models were used to evaluate the effects of greA and gre B deletion on STM pathogenicity.[Results]The mutant strains STM LT2ΔgreA and STM LT2ΔgreB were successfully constructed.Compared with the wild-type strain,the deletion of greA and gre B did not affect the cell growth rate,while inhibiting the biofilm formation,adhesion,and invasion of STM.In addition,the deletion of greA and greB decreased the colonization of STM in the liver and spleen of mice,increasing the LD50 of STM by 39.81 times and 2.5 times,respectively.[Conclusion]The deletion of greA and greB could reduce the pathogenicity of STM.This finding provides a theoretical basis for further revealing the pathogenicity of Salmonella.

关 键 词：鼠伤寒沙门菌 转录延伸因子 greA greB 生物特性 

分 类 号：S852.61[农业科学—基础兽医学]