一种核酸PCR扩增杂交一体化反应仪的设计  

Design of an Integrated PCR Hybridization Reaction Instrument for Nucleic Acid Amplification

作　　者：蔡燕敏 范嘉欢 张伟 林婉玲 刘汉旭 曾梓建 师佳乐 陈楚栋 郭继云 CAI Yanmin;FAN Jiahuan;ZHANG Wei;LIN Wanling;LIU Hanxu;ZENG Zijian;SHI Jiale;CHEN Chudong;GUO Jiyun(School of Physics and Electronic Engineering,Hanshan Normal University,Chaozhou Guangdong 521041,China;School of Life Science and Food Engineering,Hanshan Normal University,Chaozhou Guangdong 521041,China;Guangdong Capp Biotechnology Co.,Ltd.,Chaozhou Guangdong 521041,China)

机构地区：[1]韩山师范学院物理与电子工程学院,广东潮州521041 [2]韩山师范学院生命科学与食品工程学院,广东潮州521041 [3]广东凯普生物科技股份有限公司,广东潮州521041

出　　处：《微处理机》2025年第1期60-64,共5页Microprocessors

基　　金：大健康产业校企共建协同创新中心(2022凯普专项)(b22088);基于物联网的分子生物实验室自动化配套系列仪器(623013)。

摘　　要：针对传统PCR和杂交法需要使用两台仪器且存在气溶胶污染的问题,本文设计了一种核酸PCR扩增杂交一体化反应仪。该仪器通过全封闭设计将PCR扩增和杂交反应整合到同一设备中,主要由PCR扩增杂交反应芯片模块、温控模块、温度采集及控制系统、主控板和蠕动泵模块等组成。实验结果表明,该仪器具有结构简单、温度控制精度高、液体传输精准等特点,可实现自动化控制并有效避免气溶胶污染,具有良好的应用前景。To address the issues of requiring two separate instruments for traditional PCR and hybridization methods,as well as the problem of aerosol contamination,this paper presents an integrated nucleic acid PCR amplification and hybridization reactor.The device integrates PCR amplification and hybridization reactions into a single fully-enclosed system,mainly consisting of PCR amplification and hybridization reaction chip module,temperature control module,temperature acquisition and control system,main control board,and peristaltic pump module.Experimental results show that the instrument features simple structure,high temperature control precision,and accurate liquid transfer.It achieves automated control while effectively preventing aerosol contamination,demonstrating promising application prospects.

关 键 词：扩增杂交反应 反应仪 一体化反应 温控单元系统 

分 类 号：TP29[自动化与计算机技术—检测技术与自动化装置]