超高效液相色谱-串联质谱法同时测定婴幼儿配方乳粉中5种脂溶性维生素  

Simultaneous Determination of Five Fat-Soluble Vitamins in Infant Formula Milk Powder by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry

作　　者：李娇 李巧莲 周长民 袁帅 罗景阳 陈娇 丁婷 LI Jiao;LI Qiaolian;ZHOU Changmin;YUAN Shuai;LUO Jingyang;CHEN Jiao;DING Ting(Shenyang Institute of Food and Drug Control,Shenyang 110136,China;College of Food Science and Engineering,Qingdao Agricultural University,Qingdao 266109,China)

机构地区：[1]沈阳市食品药品检验所,辽宁沈阳110136 [2]青岛农业大学食品科学与工程学院,山东青岛266109

出　　处：《乳业科学与技术》2025年第1期20-23,共4页JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY

摘　　要：建立超高效液相色谱-串联质谱法同时测定婴幼儿配方乳粉中VA、VD_(3)、VK_(2)、VE和β-胡萝卜素含量。结果表明,5种脂溶性维生素在1~800 ng/mL质量浓度范围内的线性关系良好。高、中、低3个加标水平下的平均加标回收率为92.0%~108.0%,相对标准偏差为0.4%~2.6%。该方法的定量限为12.5~125μg/100 g,样品前处理步骤简单,定量分析灵敏度高,检测结果准确性和精密度良好。A method was developed for simultaneous measurement of the levels of vitamins A,D_(3),K_(2),E,andβ-carotene in infant formula milk powder using ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).The calibration curves for the five fat-soluble vitamins showed good linearity within the concentration range of 1–800 ng/mL.The average recoveries at three spiked levels were between 92.0%and 108.0%with relative standard deviations(RSDs)ranging from 0.4%to 2.6%.The limits of quantification(LOQs)of this method were 12.5–125μg/100 g.The proposed method was characterized by simple sample pre-treatment,high quantitative sensitivity,and good accuracy and precision.

关 键 词：超高效液相色谱-串联质谱法 婴幼儿配方乳粉 脂溶性维生素 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]